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. 1987 Oct 1;105(4):1799–1805. doi: 10.1083/jcb.105.4.1799

Flagellar root contraction and nuclear movement during flagellar regeneration in Chlamydomonas reinhardtii

PMCID: PMC2114663  PMID: 3667698

Abstract

When Chlamydomonas cells are deflagellated by pH shock or mechanical shear the nucleus rapidly moves toward the flagellar basal apparatus at the anterior end of the cell. During flagellar regeneration the nucleus returns to a more central position within the cell. The nucleus is connected to the flagellar apparatus by a system of fibers, the flagellar roots (rhizoplasts), which undergo a dramatic contraction that coincides with anterior nuclear movement. A corresponding extension of the root system, back to its preshock configuration is observed as the nucleus retracts to a central position. Anterior displacement of the nucleus and flagellar root contraction require free calcium in the medium. Nuclear movement and flagellar root contraction and extension are not sensitive to inhibitors of protein synthesis (cycloheximide), or drugs that influence either microtubules (colchicine) or actin-based microfilaments (cytochalasin D). Detergent- extracted cell models contract and extend their flagellar roots and move their nuclei in response to alterations of free calcium levels in the medium. Cycles of nuclear movement in detergent-extracted models require ATP to potentiate the contractile mechanism for subsequent calcium-induced contraction. Flagellar root contraction and nuclear movement in Chlamydomonas may be causally related to signaling of induction of flagellar precursor genes or to the transport of flagellar precursors or their messages to sites of synthesis or assembly near the basal apparatus of the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen N. S., Allen R. D. Cytoplasmic streaming in green plants. Annu Rev Biophys Bioeng. 1978;7:497–526. doi: 10.1146/annurev.bb.07.060178.002433. [DOI] [PubMed] [Google Scholar]
  2. Bard F., Bourgeois C. A., Costagliola D., Bouteille M. Rotation of the cell nucleus in living cells: a quantitative analysis. Biol Cell. 1985;54(2):135–142. doi: 10.1111/j.1768-322x.1985.tb00388.x. [DOI] [PubMed] [Google Scholar]
  3. Bissell M. J., Hall H. G., Parry G. How does the extracellular matrix direct gene expression? J Theor Biol. 1982 Nov 7;99(1):31–68. doi: 10.1016/0022-5193(82)90388-5. [DOI] [PubMed] [Google Scholar]
  4. Bornens M. Is the centriole bound to the nuclear membrane? Nature. 1977 Nov 3;270(5632):80–82. doi: 10.1038/270080a0. [DOI] [PubMed] [Google Scholar]
  5. De Boni U., Mintz A. H. Curvilinear, three-dimensional motion of chromatin domains and nucleoli in neuronal interphase nuclei. Science. 1986 Nov 14;234(4778):863–866. doi: 10.1126/science.3775367. [DOI] [PubMed] [Google Scholar]
  6. Lefebvre P. A., Nordstrom S. A., Moulder J. E., Rosenbaum J. L. Flagellar elongation and shortening in Chlamydomonas. IV. Effects of flagellar detachment, regeneration, and resorption on the induction of flagellar protein synthesis. J Cell Biol. 1978 Jul;78(1):8–27. doi: 10.1083/jcb.78.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lefebvre P. A., Rosenbaum J. L. Regulation of the synthesis and assembly of ciliary and flagellar proteins during regeneration. Annu Rev Cell Biol. 1986;2:517–546. doi: 10.1146/annurev.cb.02.110186.002505. [DOI] [PubMed] [Google Scholar]
  8. Nelson W. G., Pienta K. J., Barrack E. R., Coffey D. S. The role of the nuclear matrix in the organization and function of DNA. Annu Rev Biophys Biophys Chem. 1986;15:457–475. doi: 10.1146/annurev.bb.15.060186.002325. [DOI] [PubMed] [Google Scholar]
  9. Niederpruem D. J. Direct studies of dikaryotization in Schizophyllum commune. I. Live inter-cellular nuclear migration patterns. Arch Microbiol. 1980 Dec;128(2):162–171. doi: 10.1007/BF00406154. [DOI] [PubMed] [Google Scholar]
  10. Oakley B. R., Morris N. R. Nuclear movement is beta--tubulin-dependent in Aspergillus nidulans. Cell. 1980 Jan;19(1):255–262. doi: 10.1016/0092-8674(80)90407-9. [DOI] [PubMed] [Google Scholar]
  11. PETERS R. A. Hormones and the cytoskeleton. Nature. 1956 Mar 3;177(4505):426–426. doi: 10.1038/177426a0. [DOI] [PubMed] [Google Scholar]
  12. Rosenbaum J. L., Moulder J. E., Ringo D. L. Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins. J Cell Biol. 1969 May;41(2):600–619. doi: 10.1083/jcb.41.2.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SAGER R., GRANICK S. Nutritional control of sexuality in Chlamydomonas reinhardi. J Gen Physiol. 1954 Jul 20;37(6):729–742. doi: 10.1085/jgp.37.6.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Salisbury J. L., Baron A. T., Coling D. E., Martindale V. E., Sanders M. A. Calcium-modulated contractile proteins associated with the eucaryotic centrosome. Cell Motil Cytoskeleton. 1986;6(2):193–197. doi: 10.1002/cm.970060218. [DOI] [PubMed] [Google Scholar]
  15. Salisbury J. L., Baron A., Surek B., Melkonian M. Striated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle. J Cell Biol. 1984 Sep;99(3):962–970. doi: 10.1083/jcb.99.3.962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salisbury J. L., Floyd G. L. Calcium-induced contraction of the rhizoplast of a quadriflagellate green alga. Science. 1978 Dec 1;202(4371):975–977. doi: 10.1126/science.202.4371.975. [DOI] [PubMed] [Google Scholar]
  17. Scott J. A. The role of cytoskeletal integrity in cellular transformation. J Theor Biol. 1984 Jan 21;106(2):183–188. doi: 10.1016/0022-5193(84)90018-3. [DOI] [PubMed] [Google Scholar]
  18. Wang E., Cross R. K., Choppin P. W. Involvement of microtubules and 10-nm filaments in the movement and positioning of nuclei in syncytia. J Cell Biol. 1979 Nov;83(2 Pt 1):320–337. doi: 10.1083/jcb.83.2.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weeks D. P., Collis P. S. Induction of microtubule protein synthesis in Chlamydomonas reinhardi during flagellar regeneration. Cell. 1976 Sep;9(1):15–27. doi: 10.1016/0092-8674(76)90048-9. [DOI] [PubMed] [Google Scholar]
  20. Weeks D. P., Collis P., Gealt M. A. Control of induction of tubulin synthesis in Chlamydomonas reinhardi. Nature. 1977 Aug 18;268(5621):667–668. doi: 10.1038/268667a0. [DOI] [PubMed] [Google Scholar]
  21. Witman G. B., Carlson K., Berliner J., Rosenbaum J. L. Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes. J Cell Biol. 1972 Sep;54(3):507–539. doi: 10.1083/jcb.54.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wright R. L., Salisbury J., Jarvik J. W. A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation. J Cell Biol. 1985 Nov;101(5 Pt 1):1903–1912. doi: 10.1083/jcb.101.5.1903. [DOI] [PMC free article] [PubMed] [Google Scholar]

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