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. 1987 Sep 1;105(3):1047–1054. doi: 10.1083/jcb.105.3.1047

Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes

PMCID: PMC2114812  PMID: 3308902

Abstract

We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recognize antigens on the lateral transcription loops are described here. We suggest that these antigens are proteins associated with nascent RNA transcripts, although they are not among the 30-40-kD "core" heterogeneous nuclear ribonucleoproteins.

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Selected References

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