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. 1987 Nov 1;105(5):2037–2041. doi: 10.1083/jcb.105.5.2037

Polarized secretion of a platelet-derived growth factor-like chemotactic factor by endothelial cells in vitro

PMCID: PMC2114851  PMID: 3680370

Abstract

Cultured bovine aortic endothelial cells secrete a potent migration- stimulating factor for vascular smooth muscle cells (SMCs) and adventitial fibroblasts. Vascular pericytes are 20-fold less responsive, and endothelial cells themselves do not respond at all. Checkerboard analysis of SMC migration in a micro-chemotaxis chamber assay shows that the factor is chemotactic. Chemotactic activity for SMCs and adventitial fibroblasts is specifically inhibited by antibodies against platelet-derived growth factor. Endothelial cells cultured on nitrocellulose filters secrete the platelet-derived growth factor-like factor almost exclusively into the basal compartment. We suggest that this factor plays an important role in the recruitment of vascular wall cells during the morphogenesis of blood vessels and pathological conditions, such as atherosclerosis.

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Selected References

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  1. Azizkhan J., Sullivan R., Azizkhan R., Zetter B. R., Klagsbrun M. Stimulation of increased capillary endothelial cell motility by chondrosarcoma cell-derived factors. Cancer Res. 1983 Jul;43(7):3281–3286. [PubMed] [Google Scholar]
  2. Barrett T. B., Gajdusek C. M., Schwartz S. M., McDougall J. K., Benditt E. P. Expression of the sis gene by endothelial cells in culture and in vivo. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6772–6774. doi: 10.1073/pnas.81.21.6772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bernstein L. R., Antoniades H., Zetter B. R. Migration of cultured vascular cells in response to plasma and platelet-derived factors. J Cell Sci. 1982 Aug;56:71–82. doi: 10.1242/jcs.56.1.71. [DOI] [PubMed] [Google Scholar]
  4. Castellot J. J., Jr, Addonizio M. L., Rosenberg R., Karnovsky M. J. Cultured endothelial cells produce a heparinlike inhibitor of smooth muscle cell growth. J Cell Biol. 1981 Aug;90(2):372–379. doi: 10.1083/jcb.90.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Castellot J. J., Jr, Favreau L. V., Karnovsky M. J., Rosenberg R. D. Inhibition of vascular smooth muscle cell growth by endothelial cell-derived heparin. Possible role of a platelet endoglycosidase. J Biol Chem. 1982 Oct 10;257(19):11256–11260. [PubMed] [Google Scholar]
  6. DiCorleto P. E., Bowen-Pope D. F. Cultured endothelial cells produce a platelet-derived growth factor-like protein. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1919–1923. doi: 10.1073/pnas.80.7.1919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DiCorleto P. E. Cultured endothelial cells produce multiple growth factors for connective tissue cells. Exp Cell Res. 1984 Jul;153(1):167–172. doi: 10.1016/0014-4827(84)90458-0. [DOI] [PubMed] [Google Scholar]
  8. Ekblom P., Sariola H., Karkinen-Jäskeläinen M., Saxén L. The origin of the glomerular endothelium. Cell Differ. 1982 Jan;11(1):35–39. doi: 10.1016/0045-6039(82)90014-8. [DOI] [PubMed] [Google Scholar]
  9. Folkman J. How is blood vessel growth regulated in normal and neoplastic tissue? G.H.A. Clowes memorial Award lecture. Cancer Res. 1986 Feb;46(2):467–473. [PubMed] [Google Scholar]
  10. Fuller S., von Bonsdorff C. H., Simons K. Vesicular stomatitis virus infects and matures only through the basolateral surface of the polarized epithelial cell line, MDCK. Cell. 1984 Aug;38(1):65–77. doi: 10.1016/0092-8674(84)90527-0. [DOI] [PubMed] [Google Scholar]
  11. Furie M. B., Cramer E. B., Naprstek B. L., Silverstein S. C. Cultured endothelial cell monolayers that restrict the transendothelial passage of macromolecules and electrical current. J Cell Biol. 1984 Mar;98(3):1033–1041. doi: 10.1083/jcb.98.3.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gajdusek C., DiCorleto P., Ross R., Schwartz S. M. An endothelial cell-derived growth factor. J Cell Biol. 1980 May;85(2):467–472. doi: 10.1083/jcb.85.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ganz P., Davies P. F., Leopold J. A., Gimbrone M. A., Jr, Alexander R. W. Short- and long-term interactions of endothelium and vascular smooth muscle in coculture: effects on cyclic GMP production. Proc Natl Acad Sci U S A. 1986 May;83(10):3552–3556. doi: 10.1073/pnas.83.10.3552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gitlin J. D., D'Amore P. A. Culture of retinal capillary cells using selective growth media. Microvasc Res. 1983 Jul;26(1):74–80. doi: 10.1016/0026-2862(83)90056-0. [DOI] [PubMed] [Google Scholar]
  15. Grotendorst G. R., Seppä H. E., Kleinman H. K., Martin G. R. Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3669–3672. doi: 10.1073/pnas.78.6.3669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Heldin C. H., Betsholtz C., Johnsson A., Nistér M., Ek B., Rönnstrand L., Wasteson A., Westermark B. Platelet-derived growth factor: mechanism of action and relation to oncogenes. J Cell Sci Suppl. 1985;3:65–76. doi: 10.1242/jcs.1985.supplement_3.7. [DOI] [PubMed] [Google Scholar]
  17. Heldin C. H., Johnsson A., Wennergren S., Wernstedt C., Betsholtz C., Westermark B. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature. 1986 Feb 6;319(6053):511–514. doi: 10.1038/319511a0. [DOI] [PubMed] [Google Scholar]
  18. Heldin C. H., Westermark B., Wasteson A. Demonstration of an antibody against platelet-derived growth factor. Exp Cell Res. 1981 Dec;136(2):255–261. doi: 10.1016/0014-4827(81)90003-3. [DOI] [PubMed] [Google Scholar]
  19. Hughes A. F. The histogenesis of the arteries of the chick embryo. J Anat. 1943 Jul;77(Pt 4):266–287. [PMC free article] [PubMed] [Google Scholar]
  20. Jaye M., McConathy E., Drohan W., Tong B., Deuel T., Maciag T. Modulation of the sis gene transcript during endothelial cell differentiation in vitro. Science. 1985 May 17;228(4701):882–885. doi: 10.1126/science.3890179. [DOI] [PubMed] [Google Scholar]
  21. Jotereau F. V., Le Douarin N. M. The development relationship between osteocytes and osteoclasts: a study using the quail-chick nuclear marker in endochondral ossification. Dev Biol. 1978 Apr;63(2):253–265. doi: 10.1016/0012-1606(78)90132-x. [DOI] [PubMed] [Google Scholar]
  22. Le Douarin N. M. Cell migrations in embryos. Cell. 1984 Sep;38(2):353–360. doi: 10.1016/0092-8674(84)90490-2. [DOI] [PubMed] [Google Scholar]
  23. Le Lièvre C. S., Le Douarin N. M. Mesenchymal derivatives of the neural crest: analysis of chimaeric quail and chick embryos. J Embryol Exp Morphol. 1975 Aug;34(1):125–154. [PubMed] [Google Scholar]
  24. Liotta L. A., Mandler R., Murano G., Katz D. A., Gordon R. K., Chiang P. K., Schiffmann E. Tumor cell autocrine motility factor. Proc Natl Acad Sci U S A. 1986 May;83(10):3302–3306. doi: 10.1073/pnas.83.10.3302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Muller W. A., Gimbrone M. A., Jr Plasmalemmal proteins of cultured vascular endothelial cells exhibit apical-basal polarity: analysis by surface-selective iodination. J Cell Biol. 1986 Dec;103(6 Pt 1):2389–2402. doi: 10.1083/jcb.103.6.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Quinn M. T., Parthasarathy S., Steinberg D. Endothelial cell-derived chemotactic activity for mouse peritoneal macrophages and the effects of modified forms of low density lipoprotein. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5949–5953. doi: 10.1073/pnas.82.17.5949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Read S. M., Northcote D. H. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem. 1981 Sep 1;116(1):53–64. doi: 10.1016/0003-2697(81)90321-3. [DOI] [PubMed] [Google Scholar]
  28. Risau W. Developing brain produces an angiogenesis factor. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3855–3859. doi: 10.1073/pnas.83.11.3855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Risau W., Ekblom P. Production of a heparin-binding angiogenesis factor by the embryonic kidney. J Cell Biol. 1986 Sep;103(3):1101–1107. doi: 10.1083/jcb.103.3.1101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ross R., Raines E. W., Bowen-Pope D. F. The biology of platelet-derived growth factor. Cell. 1986 Jul 18;46(2):155–169. doi: 10.1016/0092-8674(86)90733-6. [DOI] [PubMed] [Google Scholar]
  31. Ross R. The pathogenesis of atherosclerosis--an update. N Engl J Med. 1986 Feb 20;314(8):488–500. doi: 10.1056/NEJM198602203140806. [DOI] [PubMed] [Google Scholar]
  32. Schwartz S. M. Selection and characterization of bovine aortic endothelial cells. In Vitro. 1978 Dec;14(12):966–980. doi: 10.1007/BF02616210. [DOI] [PubMed] [Google Scholar]
  33. Seppä H., Grotendorst G., Seppä S., Schiffmann E., Martin G. R. Platelet-derived growth factor in chemotactic for fibroblasts. J Cell Biol. 1982 Feb;92(2):584–588. doi: 10.1083/jcb.92.2.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Simionescu M., Simionescu N., Palade G. E. Preferential distribution of anionic sites on the basement membrane and the abluminal aspect of the endothelium in fenestrated capillaries. J Cell Biol. 1982 Nov;95(2 Pt 1):425–434. doi: 10.1083/jcb.95.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Terranova V. P., DiFlorio R., Lyall R. M., Hic S., Friesel R., Maciag T. Human endothelial cells are chemotactic to endothelial cell growth factor and heparin. J Cell Biol. 1985 Dec;101(6):2330–2334. doi: 10.1083/jcb.101.6.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. van Buul-Wortelboer M. F., Brinkman H. J., Dingemans K. P., de Groot P. G., van Aken W. G., van Mourik J. A. Reconstitution of the vascular wall in vitro. A novel model to study interactions between endothelial and smooth muscle cells. Exp Cell Res. 1986 Jan;162(1):151–158. doi: 10.1016/0014-4827(86)90433-7. [DOI] [PubMed] [Google Scholar]

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