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TPA induction is blocked by PKC and MEK1 inhibition. Total RNA isolated from the stably transfected clonal cell line HS2(2.2)-1, treated as indicated, was analyzed by Northern blotting. Cells were exposed to inhibitors or vehicle for 2.5 h. For those treatments in which TPA was included, TPA was present during the final 2 h of the treatment. Luciferase mRNA levels were quantified and normalized to those encoding glyceraldehyde-3-phosphate dehydrogenase.
