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. 1988 Oct;170(10):4823–4827. doi: 10.1128/jb.170.10.4823-4827.1988

Activation of protease-constitutive recA proteins of Escherichia coli by rRNA and tRNA.

W B Wang 1, E S Tessman 1, I Tessman 1
PMCID: PMC211526  PMID: 2459110

Abstract

The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater than RecA1211 much greater than RecA+, which is also the order of their in vivo constitutive protease activities. The effectiveness of rRNA was comparable to that of ssDNA in the cleavage of the LexA repressor by either mutant protease. Although all the common nucleoside triphosphates can act as positive effectors for LexA cleavage by the two mutant proteins in the presence of ssDNA (W. B. Wang, M. Sassanfar, I. Tessman, J. W. Roberts, and E. S. Tessman, J. Bacteriol. 170:4816-4822, 1988), only dATP, ATP, and ATP-gamma-S were effective in the presence of RNA. Our results explain more fully why certain recA mutants have high constitutive protease activities in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Craig N. L., Roberts J. W. E. coli recA protein-directed cleavage of phage lambda repressor requires polynucleotide. Nature. 1980 Jan 3;283(5742):26–30. doi: 10.1038/283026a0. [DOI] [PubMed] [Google Scholar]
  2. Craig N. L., Roberts J. W. Function of nucleoside triphosphate and polynucleotide in Escherichia coli recA protein-directed cleavage of phage lambda repressor. J Biol Chem. 1981 Aug 10;256(15):8039–8044. [PubMed] [Google Scholar]
  3. D'Ari R., Huisman O. DNA replication and indirect induction of the SOS response in Escherichia coli. Biochimie. 1982 Aug-Sep;64(8-9):623–627. doi: 10.1016/s0300-9084(82)80100-4. [DOI] [PubMed] [Google Scholar]
  4. GOLDTHWAIT D., JACOB F. SUR LE M'ECANISME DE L'INDUCTION DU D'EVELOPPEMENT DU PROPHAGE CHEZ LES BACT'ERIES LYSOG'ENES. C R Hebd Seances Acad Sci. 1964 Jul 20;259:661–664. [PubMed] [Google Scholar]
  5. Kowalczykowski S. C. Interaction of recA protein with a photoaffinity analogue of ATP, 8-azido-ATP: determination of nucleotide cofactor binding parameters and of the relationship between ATP binding and ATP hydrolysis. Biochemistry. 1986 Oct 7;25(20):5872–5881. doi: 10.1021/bi00368a006. [DOI] [PubMed] [Google Scholar]
  6. Little J. W. Autodigestion of lexA and phage lambda repressors. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1375–1379. doi: 10.1073/pnas.81.5.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Little J. W., Mount D. W. The SOS regulatory system of Escherichia coli. Cell. 1982 May;29(1):11–22. doi: 10.1016/0092-8674(82)90085-x. [DOI] [PubMed] [Google Scholar]
  8. Lu C., Echols H. RecA protein and SOS. Correlation of mutagenesis phenotype with binding of mutant RecA proteins to duplex DNA and LexA cleavage. J Mol Biol. 1987 Aug 5;196(3):497–504. doi: 10.1016/0022-2836(87)90027-1. [DOI] [PubMed] [Google Scholar]
  9. Lu C., Scheuermann R. H., Echols H. Capacity of RecA protein to bind preferentially to UV lesions and inhibit the editing subunit (epsilon) of DNA polymerase III: a possible mechanism for SOS-induced targeted mutagenesis. Proc Natl Acad Sci U S A. 1986 Feb;83(3):619–623. doi: 10.1073/pnas.83.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McEntee K., Weinstock G. M. tif-1 mutation alters polynucleotide recognition by the recA protein of Escherichia coli. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6061–6065. doi: 10.1073/pnas.78.10.6061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Phizicky E. M., Roberts J. W. Induction of SOS functions: regulation of proteolytic activity of E. coli RecA protein by interaction with DNA and nucleoside triphosphate. Cell. 1981 Jul;25(1):259–267. doi: 10.1016/0092-8674(81)90251-8. [DOI] [PubMed] [Google Scholar]
  12. Radding C. M. Homologous pairing and strand exchange in genetic recombination. Annu Rev Genet. 1982;16:405–437. doi: 10.1146/annurev.ge.16.120182.002201. [DOI] [PubMed] [Google Scholar]
  13. Register J. C., 3rd, Griffith J. 10 nm RecA protein filaments formed in the presence of Mg2+ and ATP gamma S may contain RNA. Mol Gen Genet. 1985;199(3):415–420. doi: 10.1007/BF00330752. [DOI] [PubMed] [Google Scholar]
  14. Roberts J. W., Phizicky E. M., Burbee D. G., Roberts C. W., Moreau P. L. A brief consideration of the SOS inducing signal. Biochimie. 1982 Aug-Sep;64(8-9):805–807. doi: 10.1016/s0300-9084(82)80133-8. [DOI] [PubMed] [Google Scholar]
  15. Ruff W., Kirby E. P., Goldthwait D. A. Cell division and prophage induction in Escherichia coli: studies of nucleotide levels. J Bacteriol. 1971 Jun;106(3):994–1004. doi: 10.1128/jb.106.3.994-1004.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slilaty S. N., Rupley J. A., Little J. W. Intramolecular cleavage of LexA and phage lambda repressors: dependence of kinetics on repressor concentration, pH, temperature, and solvent. Biochemistry. 1986 Nov 4;25(22):6866–6875. doi: 10.1021/bi00370a020. [DOI] [PubMed] [Google Scholar]
  17. Smith K. C., Meun D. H. Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on post-replication repair of damage due to ultraviolet radiation. J Mol Biol. 1970 Aug;51(3):459–472. doi: 10.1016/0022-2836(70)90001-x. [DOI] [PubMed] [Google Scholar]
  18. Tessman E. S., Peterson P. K. Isolation of protease-proficient, recombinase-deficient recA mutants of Escherichia coli K-12. J Bacteriol. 1985 Aug;163(2):688–695. doi: 10.1128/jb.163.2.688-695.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tessman E. S., Peterson P. Plaque color method for rapid isolation of novel recA mutants of Escherichia coli K-12: new classes of protease-constitutive recA mutants. J Bacteriol. 1985 Aug;163(2):677–687. doi: 10.1128/jb.163.2.677-687.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tessman E. S., Tessman I., Peterson P. K., Forestal J. D. Roles of RecA protease and recombinase activities of Escherichia coli in spontaneous and UV-induced mutagenesis and in Weigle repair. J Bacteriol. 1986 Dec;168(3):1159–1164. doi: 10.1128/jb.168.3.1159-1164.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Walker G. C. Inducible DNA repair systems. Annu Rev Biochem. 1985;54:425–457. doi: 10.1146/annurev.bi.54.070185.002233. [DOI] [PubMed] [Google Scholar]
  22. Walker G. C. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984 Mar;48(1):60–93. doi: 10.1128/mr.48.1.60-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wang W. B., Sassanfar M., Tessman I., Roberts J. W., Tessman E. S. Activation of protease-constitutive recA proteins of Escherichia coli by all of the common nucleoside triphosphates. J Bacteriol. 1988 Oct;170(10):4816–4822. doi: 10.1128/jb.170.10.4816-4822.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wang W. B., Tessman E. S. Location of functional regions of the Escherichia coli RecA protein by DNA sequence analysis of RecA protease-constitutive mutants. J Bacteriol. 1986 Nov;168(2):901–910. doi: 10.1128/jb.168.2.901-910.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weinstock G. M., McEntee K. RecA protein-dependent proteolysis of bacteriophage lambda repressor Characterization of the reaction and stimulation by DNA-binding proteins. J Biol Chem. 1981 Nov 10;256(21):10883–10888. [PubMed] [Google Scholar]
  26. Witkin E. M. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. doi: 10.1128/br.40.4.869-907.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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