Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Nov;170(11):5364–5367. doi: 10.1128/jb.170.11.5364-5367.1988

Isolation and characterization of Pseudomonas aeruginosa mutants requiring salicylic acid for pyochelin biosynthesis.

R G Ankenbauer 1, C D Cox 1
PMCID: PMC211614  PMID: 3141387

Abstract

Pseudomonas aeruginosa mutants requiring salicylic acid for pyochelin biosynthesis were isolated after chemical mutagenesis by plating on a siderophore detection medium. Like the wild type, these mutants incorporated 7-[14C]salicylic acid into pyochelin, demonstrating that salicylic acid is an intermediate in the biosynthesis pathway of pyochelin.

Full text

PDF
5364

Images in this article

5365Image
on p.5365

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ankenbauer R. G., Toyokuni T., Staley A., Rinehart K. L., Jr, Cox C. D. Synthesis and biological activity of pyochelin, a siderophore of Pseudomonas aeruginosa. J Bacteriol. 1988 Nov;170(11):5344–5351. doi: 10.1128/jb.170.11.5344-5351.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ankenbauer R., Hanne L. F., Cox C. D. Mapping of mutations in Pseudomonas aeruginosa defective in pyoverdin production. J Bacteriol. 1986 Jul;167(1):7–11. doi: 10.1128/jb.167.1.7-11.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ankenbauer R., Sriyosachati S., Cox C. D. Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin. Infect Immun. 1985 Jul;49(1):132–140. doi: 10.1128/iai.49.1.132-140.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Briskot G., Taraz K., Budzikiewicz H. Siderophore vom Pyoverdin-Typ aus Pseudomonas aeruginosa. Z Naturforsch C. 1986 May-Jun;41(5-6):497–506. [PubMed] [Google Scholar]
  5. Cox C. D., Adams P. Siderophore activity of pyoverdin for Pseudomonas aeruginosa. Infect Immun. 1985 Apr;48(1):130–138. doi: 10.1128/iai.48.1.130-138.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cox C. D. Effect of pyochelin on the virulence of Pseudomonas aeruginosa. Infect Immun. 1982 Apr;36(1):17–23. doi: 10.1128/iai.36.1.17-23.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cox C. D., Graham R. Isolation of an iron-binding compound from Pseudomonas aeruginosa. J Bacteriol. 1979 Jan;137(1):357–364. doi: 10.1128/jb.137.1.357-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cox C. D. Iron transport and serum resistance in Pseudomonas aeruginosa. Antibiot Chemother (1971) 1985;36:1–12. doi: 10.1159/000410466. [DOI] [PubMed] [Google Scholar]
  9. Cox C. D., Rinehart K. L., Jr, Moore M. L., Cook J. C., Jr Pyochelin: novel structure of an iron-chelating growth promoter for Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4256–4260. doi: 10.1073/pnas.78.7.4256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FRANK L. H., DEMOSS R. D. On the biosynthesis of pyocyanine. J Bacteriol. 1959 Jun;77(6):776–782. doi: 10.1128/jb.77.6.776-782.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goldberg J. B., Ohman D. E. Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate. J Bacteriol. 1984 Jun;158(3):1115–1121. doi: 10.1128/jb.158.3.1115-1121.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hudson A. T., Bentley R. Utilization of shikimic acid for the formation of mycobactin S and salicylic acid by Mycobacterium smegmatis. Biochemistry. 1970 Sep 29;9(20):3984–3987. doi: 10.1021/bi00822a017. [DOI] [PubMed] [Google Scholar]
  13. Ingledew W. M., Campbell J. J. A new resuspension medium for pyocyanine production. Can J Microbiol. 1969 Jun;15(6):595–598. doi: 10.1139/m69-101. [DOI] [PubMed] [Google Scholar]
  14. LIN E. C., LERNER S. A., JORGENSEN S. E. A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes. Biochim Biophys Acta. 1962 Jul 2;60:422–424. doi: 10.1016/0006-3002(62)90423-7. [DOI] [PubMed] [Google Scholar]
  15. Ratledge C., Hall M. J. Isolation and properties of auxotrophic mutants of Mycobacterium smegmatis requiring either salicylic acid or mycobactin. J Gen Microbiol. 1972 Aug;72(1):143–150. doi: 10.1099/00221287-72-1-143. [DOI] [PubMed] [Google Scholar]
  16. Ratledge C., Macham L. P., Brown K. A., Marshall B. J. Iron transport in Mycobacterium smegmatis: a restricted role for salicylic acid in the extracellular environment. Biochim Biophys Acta. 1974 Nov 4;372(1):39–51. doi: 10.1016/0304-4165(74)90071-3. [DOI] [PubMed] [Google Scholar]
  17. Schwyn B., Neilands J. B. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987 Jan;160(1):47–56. doi: 10.1016/0003-2697(87)90612-9. [DOI] [PubMed] [Google Scholar]
  18. Sokol P. A. Production and utilization of pyochelin by clinical isolates of Pseudomonas cepacia. J Clin Microbiol. 1986 Mar;23(3):560–562. doi: 10.1128/jcm.23.3.560-562.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sokol P. A. Surface expression of ferripyochelin-binding protein is required for virulence of Pseudomonas aeruginosa. Infect Immun. 1987 Sep;55(9):2021–2025. doi: 10.1128/iai.55.9.2021-2025.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sriyosachati S., Cox C. D. Siderophore-mediated iron acquisition from transferrin by Pseudomonas aeruginosa. Infect Immun. 1986 Jun;52(3):885–891. doi: 10.1128/iai.52.3.885-891.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Teintze M., Hossain M. B., Barnes C. L., Leong J., van der Helm D. Structure of ferric pseudobactin, a siderophore from a plant growth promoting Pseudomonas. Biochemistry. 1981 Oct 27;20(22):6446–6457. doi: 10.1021/bi00525a025. [DOI] [PubMed] [Google Scholar]
  22. Turner J. M., Messenger A. J. Occurrence, biochemistry and physiology of phenazine pigment production. Adv Microb Physiol. 1986;27:211–275. doi: 10.1016/s0065-2911(08)60306-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES