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. 1990 Aug 1;111(2):401–407. doi: 10.1083/jcb.111.2.401

A rapid posttranslational myristylation of a 68-kD protein in D. discoideum

PMCID: PMC2116220  PMID: 2199457

Abstract

Cells incubated with [3H]myristate were shown to rapidly and specifically acylate a 68-kD protein, p68, in a developmentally- regulated manner. The fatty acid incorporated into p68 was identified as myristate, and is linked to the protein via an amide bond, apparently to an NH2-terminal glycine. The acylation of p68 in D. discoideum displays some unusual properties. Unexpectedly, myristylation of p68 is a posttranslational event and occurs in the presence of inhibitors of protein synthesis. Another unusual finding was that although p68 is a stable protein, the acyl moiety is removed with a half time of approximately 15 min.

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Selected References

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  1. Aveldaño M. I., Horrocks L. A. Quantitative release of fatty acids from lipids by a simple hydrolysis procedure. J Lipid Res. 1983 Aug;24(8):1101–1105. [PubMed] [Google Scholar]
  2. Beug H., Katz F. E., Gerisch G. Dynamics of antigenic membrane sites relating to cell aggregation in Dictyostelium discoideum. J Cell Biol. 1973 Mar;56(3):647–658. doi: 10.1083/jcb.56.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cross G. A. Eukaryotic protein modification and membrane attachment via phosphatidylinositol. Cell. 1987 Jan 30;48(2):179–181. doi: 10.1016/0092-8674(87)90419-3. [DOI] [PubMed] [Google Scholar]
  4. Grand R. J. Acylation of viral and eukaryotic proteins. Biochem J. 1989 Mar 15;258(3):625–638. doi: 10.1042/bj2580625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hedo J. A., Collier E., Watkinson A. Myristyl and palmityl acylation of the insulin receptor. J Biol Chem. 1987 Jan 25;262(3):954–957. [PubMed] [Google Scholar]
  6. Juliani M. H., Brusca J., Klein C. cAMP regulation of cell differentiation in Dictyostelium discoideum and the role of the cAMP receptor. Dev Biol. 1981 Apr 15;83(1):114–121. doi: 10.1016/s0012-1606(81)80013-9. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Loomis W. F., Wheeler S. A., Springer W. R., Barondes S. H. Adhesion mutants of Dictyostelium discoideum lacking the saccharide determinant recognized by two adhesion-blocking monoclonal antibodies. Dev Biol. 1985 May;109(1):111–117. doi: 10.1016/0012-1606(85)90351-3. [DOI] [PubMed] [Google Scholar]
  9. Meier K., Klein C. An unusual protein kinase phosphorylates the chemotactic receptor of Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2181–2185. doi: 10.1073/pnas.85.7.2181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Monteiro M., Magee A. I. Fatty acid acylation of proteins in Physarum polycephalum. Cell Biol Int Rep. 1987 Sep;11(9):645–650. doi: 10.1016/0309-1651(87)90098-1. [DOI] [PubMed] [Google Scholar]
  11. Ochiai H., Stadler J., Westphal M., Wagle G., Merkl R., Gerisch G. Monoclonal antibodies against contact sites A of Dictyostelium discoideum: detection of modifications of the glycoprotein in tunicamycin-treated cells. EMBO J. 1982;1(8):1011–1016. doi: 10.1002/j.1460-2075.1982.tb01286.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olson E. N., Towler D. A., Glaser L. Specificity of fatty acid acylation of cellular proteins. J Biol Chem. 1985 Mar 25;260(6):3784–3790. [PubMed] [Google Scholar]
  13. Pillai S., Baltimore D. Myristoylation and the post-translational acquisition of hydrophobicity by the membrane immunoglobulin heavy-chain polypeptide in B lymphocytes. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7654–7658. doi: 10.1073/pnas.84.21.7654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ryals P. E., Thompson G. A., Jr Protein acylation in Tetrahymena. Arch Biochem Biophys. 1988 Nov 1;266(2):408–415. doi: 10.1016/0003-9861(88)90272-x. [DOI] [PubMed] [Google Scholar]
  15. Sadeghi H., da Silva A. M., Klein C. Evidence that a glycolipid tail anchors antigen 117 to the plasma membrane of Dictyostelium discoideum cells. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5512–5515. doi: 10.1073/pnas.85.15.5512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schultz A. M., Henderson L. E., Oroszlan S. Fatty acylation of proteins. Annu Rev Cell Biol. 1988;4:611–647. doi: 10.1146/annurev.cb.04.110188.003143. [DOI] [PubMed] [Google Scholar]
  17. Schultz A., Oroszlan S. Myristylation of gag-onc fusion proteins in mammalian transforming retroviruses. Virology. 1984 Mar;133(2):431–437. doi: 10.1016/0042-6822(84)90409-4. [DOI] [PubMed] [Google Scholar]
  18. Sefton B. M., Buss J. E. The covalent modification of eukaryotic proteins with lipid. J Cell Biol. 1987 Jun;104(6):1449–1453. doi: 10.1083/jcb.104.6.1449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stadler J., Gerisch G., Bauer G., Deppert W. In vivo acylation of Dictyostelium actin with palmitic acid. EMBO J. 1985 May;4(5):1153–1156. doi: 10.1002/j.1460-2075.1985.tb03753.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stadler J., Keenan T. W., Bauer G., Gerisch G. The contact site A glycoprotein of Dictyostelium discoideum carries a phospholipid anchor of a novel type. EMBO J. 1989 Feb;8(2):371–377. doi: 10.1002/j.1460-2075.1989.tb03387.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Towler D. A., Gordon J. I., Adams S. P., Glaser L. The biology and enzymology of eukaryotic protein acylation. Annu Rev Biochem. 1988;57:69–99. doi: 10.1146/annurev.bi.57.070188.000441. [DOI] [PubMed] [Google Scholar]
  22. Towler D., Glaser L. Acylation of cellular proteins with endogenously synthesized fatty acids. Biochemistry. 1986 Feb 25;25(4):878–884. doi: 10.1021/bi00352a021. [DOI] [PubMed] [Google Scholar]
  23. Vijayasarathy C., Bhat N. R., Avadhani N. G. Intramitochondrial fatty acylation of a cytoplasmic imported protein in animal cells. J Biol Chem. 1989 May 15;264(14):7772–7775. [PubMed] [Google Scholar]
  24. Watts D. J., Ashworth J. M. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. doi: 10.1042/bj1190171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weeks G., Lima A. F., Pawson T. A ras-encoded protein in Dictyostelium discoideum is acylated and membrane-associated. Mol Microbiol. 1987 Nov;1(3):347–354. doi: 10.1111/j.1365-2958.1987.tb01941.x. [DOI] [PubMed] [Google Scholar]

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