Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Dec;170(12):5935–5938. doi: 10.1128/jb.170.12.5935-5938.1988

Nucleotide sequence of the Bacillus subtilis phoR gene.

T Seki 1, H Yoshikawa 1, H Takahashi 1, H Saito 1
PMCID: PMC211708  PMID: 3142862

Abstract

The nucleotide sequence of phoR, the positive and negative regulatory gene for alkaline phosphatase and phosphodiesterase formation in Bacillus subtilis, was determined. The sequence data predicted an open reading frame of 1,740 base pairs (579 amino acids) which overlaps the 5 base pairs of the preceding phoP coding sequence. The deduced amino acid sequence was significantly homologous with that of the Escherichia coli phoR gene product, which is the sensory element for the pho regulon.

Full text

PDF
5935

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albin R., Weber R., Silverman P. M. The Cpx proteins of Escherichia coli K12. Immunologic detection of the chromosomal cpxA gene product. J Biol Chem. 1986 Apr 5;261(10):4698–4705. [PubMed] [Google Scholar]
  2. Comeau D. E., Ikenaka K., Tsung K. L., Inouye M. Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol. 1985 Nov;164(2):578–584. doi: 10.1128/jb.164.2.578-584.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  4. Leroux B., Yanofsky M. F., Winans S. C., Ward J. E., Ziegler S. F., Nester E. W. Characterization of the virA locus of Agrobacterium tumefaciens: a transcriptional regulator and host range determinant. EMBO J. 1987 Apr;6(4):849–856. doi: 10.1002/j.1460-2075.1987.tb04830.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MacFarlane S. A., Merrick M. The nucleotide sequence of the nitrogen regulation gene ntrB and the glnA-ntrBC intergenic region of Klebsiella pneumoniae. Nucleic Acids Res. 1985 Nov 11;13(21):7591–7606. doi: 10.1093/nar/13.21.7591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Makino K., Shinagawa H., Amemura M., Nakata A. Nucleotide sequence of the phoR gene, a regulatory gene for the phosphate regulon of Escherichia coli. J Mol Biol. 1986 Dec 5;192(3):549–556. doi: 10.1016/0022-2836(86)90275-5. [DOI] [PubMed] [Google Scholar]
  7. Makino K., Shinagawa H., Nakata A. Regulation of the phosphate regulon of Escherichia coli K-12: regulation and role of the regulatory gene phoR. J Mol Biol. 1985 Jul 20;184(2):231–240. doi: 10.1016/0022-2836(85)90376-6. [DOI] [PubMed] [Google Scholar]
  8. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  9. Miki T., Minami Z., Ikeda Y. The genetics of alkaline phosphatase formation in Bacillus subtilis. Genetics. 1965 Nov;52(5):1093–1100. doi: 10.1093/genetics/52.5.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nixon B. T., Ronson C. W., Ausubel F. M. Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7850–7854. doi: 10.1073/pnas.83.20.7850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nukushina J. I., Ikeda Y. Genetic analysis of the developmental processes during germination and outgrowth of Bacillus subtilis spores with temperature-sensitive mutants. Genetics. 1969 Sep;63(1):63–74. doi: 10.1093/genetics/63.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Piggot P. J., Hoch J. A. Revised genetic linkage map of Bacillus subtilis. Microbiol Rev. 1985 Jun;49(2):158–179. doi: 10.1128/mr.49.2.158-179.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Piggot P. J., Taylor S. Y. New types of mutation affecting formation of alkaline phosphatase by Bacillus subtilis in sporulation conditions. J Gen Microbiol. 1977 Sep;102(1):69–80. doi: 10.1099/00221287-102-1-69. [DOI] [PubMed] [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seki T., Yoshikawa H., Takahashi H., Saito H. Cloning and nucleotide sequence of phoP, the regulatory gene for alkaline phosphatase and phosphodiesterase in Bacillus subtilis. J Bacteriol. 1987 Jul;169(7):2913–2916. doi: 10.1128/jb.169.7.2913-2916.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wanner B. L., Chang B. D. The phoBR operon in Escherichia coli K-12. J Bacteriol. 1987 Dec;169(12):5569–5574. doi: 10.1128/jb.169.12.5569-5574.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yamane K., Maruo B. Alkaline phosphatase possessing alkaline phosphodiesterase activity and other phosphodiesterases in Bacillus subtilis. J Bacteriol. 1978 Apr;134(1):108–114. doi: 10.1128/jb.134.1.108-114.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES