Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Jan;169(1):137–141. doi: 10.1128/jb.169.1.137-141.1987

Cell surface polysaccharides from Bradyrhizobium japonicum and a nonnodulating mutant.

V Puvanesarajah, F M Schell, D Gerhold, G Stacey
PMCID: PMC211745  PMID: 3793715

Abstract

The cell surface polysaccharides of wild-type Bradyrhizobium japonicum USDA 110 and a nonnodulating mutant, strain HS123, were analyzed. The capsular polysaccharide (CPS) and exopolysaccharide (EPS) of the wild type and the mutant strain do not differ in their sugar composition. CPS and EPS are composed of mannose, 4-O-methylgalactose/galactose, glucose, and galacturonic acid in a ratio of 1:1:2:1, respectively. H nuclear magnetic resonance spectra of the EPS and CPS of the wild type and mutant strain are very similar, but not identical, suggesting minor structural variation in these polysaccharides. The lipopolysaccharides (LPS) of the above two strains were purified, and their compositions were determined. Gross differences in the chemical compositions of the two LPS were observed. Chemical and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses indicated that strain HS123 is a rough-type mutant lacking a complete LPS. The LPS of mutant strain HS123 is composed of mannose, glucose, glucosamine, 2-keto-3-deoxyoctulosonic acid, and lipid A. The wild-type LPS is composed of fucose, xylose, arabinose, mannose, glucose, fucosamine, quinovosamine, glucosamine, uronic acid, 2-keto-3-deoxyoctulosonic acid, and lipid A. Preliminary sugar analysis of lipid A from B. japonicum identified mannose, while traces of glucosamine were detected. 3-Hydroxydodecanoic and 3-hydroxytetradecanoic acids formed a major portion of the fatty acids in lipid A. Lesser quantities of nonhydroxylated 16:0, 18:0, 22:0, and 24:0 acids also were detected.

Full text

PDF
137

Images in this article

140Image
on p.140

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
  2. Carlson R. W., Sanders R. E., Napoli C., Albersheim P. Host-Symbiont Interactions: III. Purification and Partial Characterization of Rhizobium Lipopolysaccharides. Plant Physiol. 1978 Dec;62(6):912–917. doi: 10.1104/pp.62.6.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edlund A., Nichols P. D., Roffey R., White D. C. Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species. J Lipid Res. 1985 Aug;26(8):982–988. [PubMed] [Google Scholar]
  4. Holst O., Borowiak D., Weckesser J., Mayer H. Structural studies on the phosphate-free lipid A of Rhodomicrobium vannielii ATCC 17100. Eur J Biochem. 1983 Dec 1;137(1-2):325–332. doi: 10.1111/j.1432-1033.1983.tb07832.x. [DOI] [PubMed] [Google Scholar]
  5. Johnson K. G., Perry M. B. Improved techniques for the preparation of bacterial lipopolysaccharides. Can J Microbiol. 1976 Jan;22(1):29–34. doi: 10.1139/m76-004. [DOI] [PubMed] [Google Scholar]
  6. Karkhanis Y. D., Zeltner J. Y., Jackson J. J., Carlo D. J. A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem. 1978 Apr;85(2):595–601. doi: 10.1016/0003-2697(78)90260-9. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Maier R. C., Norris H. J. Glassy cell carcinoma of the cervix. Obstet Gynecol. 1982 Aug;60(2):219–224. [PubMed] [Google Scholar]
  9. Maier R. J., Brill W. J. Involvement of Rhizobium japonicum O antigen in soybean nodulation. J Bacteriol. 1978 Mar;133(3):1295–1299. doi: 10.1128/jb.133.3.1295-1299.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mort A. J., Bauer W. D. Application of two new methods for cleavage of polysaccharides into specific oligosaccharide fragments. Structure of the capsular and extracellular polysaccharides of Rhizobium japonicum that bind soybean lectin. J Biol Chem. 1982 Feb 25;257(4):1870–1875. [PubMed] [Google Scholar]
  11. Mort A. J., Bauer W. D. Composition of the Capsular and Extracellular Polysaccharides of Rhizobium japonicum: CHANGES WITH CULTURE AGE AND CORRELATIONS WITH BINDING OF SOYBEAN SEED LECTIN TO THE BACTERIA . Plant Physiol. 1980 Jul;66(1):158–163. doi: 10.1104/pp.66.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peterson A. A., McGroarty E. J. High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli. J Bacteriol. 1985 May;162(2):738–745. doi: 10.1128/jb.162.2.738-745.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pueppke S. G. Adsorption of slow- and fast-growing rhizobia to soybean and cowpea roots. Plant Physiol. 1984 Aug;75(4):924–928. doi: 10.1104/pp.75.4.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stacey G., Pocratsky L. A., Puvanesarajah V. Bacteriophage that can distinguish between wild-type Rhizobium japonicum and a non-nodulating mutant. Appl Environ Microbiol. 1984 Jul;48(1):68–72. doi: 10.1128/aem.48.1.68-72.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES