Vol. 203, No. 1, January 23, 2006. Pages 215–226.
The original version of this article contained the following production errors:
In the third paragraph of the introduction, a “c” erroneously appeared before the word “complexes.” The sentence should have read:
“Biochemical studies indicate that a modified form of AID from B cells interacts with replication protein A (RPA) and that AID–RPA complexes specifically bind to ssDNA located in transcription bubbles at DGYW motifs and deaminate DNA (16).”
In the first sentence of the Fig. 6 legend, the word “in” erroneously replaced the word “is.” The sentence should have read:
“Histone H3 and H4 Ac is significantly reduced across the γ3 locus in LPS activated AID KO B cells.”
In Table I, the VH/JH3 forward primer sequence should have read GGAAAGGGTCTGGAGTGGCT. Additionally, the alignment of forward and reverse primers in the β-globin row shifted upward. The corrected table appears below.
Table I.
Primers used in chromatin immunoprecipitation assays
| Amplicon | Forward primer | Reverse primer |
|---|---|---|
| SμDS | CTGAATGAGTTTCACCAGGCC | GCCTGTCCTGCTTGGCTTC |
| Iγ3UP | GGCCCCTTGAACCAAACCT | TCAGTCTCACCTGCATGGTCAT |
| Iγ3-P | CACTCACTCTTGCCTTCTCCTACAT | GGGGGAAAACTGGTACAGCAGTGTG |
| Iγ3Ex | AGATCCCAAAGCTAAGCTCCTG | CAGAGAGACCCCCTCCACAGT |
| Iγ3Ex-D | GCCAAGAGTGTGGAACTCTAAGGT | GTGCCTCAGTACCTCTCAGCTG |
| Sγ3-U | GCTGAGAGTATGCACAGCCA | GGATCATGGAAACTCCTCCG |
| Sγ3-D | ACAGGGTCCCAGGTTATGCAG | GTCACTCACACTGGCTTCCC |
| Cγ3-U | GTTCCCATTAGAAAAATACC | CTGCTAGATTCTGCACTGAC |
| Cγ3-D | ATGGTGACCACGAGGGAGAG | CACCTTGGGAAGGCAGTGAGA |
| Iγ1-P | GAGTCTCAAGGGTACCGCCTCA | CAGAGTCACAGCCTTTGTCCCA |
| Sγ1-U | GGAGGAGATATCCAAGAGCA | CAGCTCTTTTGCAGGTCTTG |
| Sγ1-D | CAGGCAAACTAAACCAGTGGG | AGGATGTCCACCCTCACCCAGGC |
| Iɛ-P | GCCTCAGGGCAGTGACACTC | GCCTCAGTGCCCTCAGCTAA |
| Sɛ-U | AGGAGAGGCTGGAGAGGAATCG | AGGGCAGCACAGATCCCACC |
| Sɛ-D | AGCTGAGCCCCTCTCTCAGG | CACTCACTGCCCCTAGCTGGCT |
| VH/JH3 | GGAAAGGGTCTGGAGTGGCT | CCTTGGCCCCAGTAAGCAA |
| β-globin | GCCTTGCCTGTTCCTGCTC | CAGACCATAAACTGTATTTTTCTTATTGAGCCC |
| Mb-1 | CCACGCACTAGAGAGAGACTCAA | CCGCCTCACTTCCTGTTCAGCCG |