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. 1987 Feb;169(2):619–623. doi: 10.1128/jb.169.2.619-623.1987

Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO.

Y Yoshioka, H Ohtsubo, E Ohtsubo
PMCID: PMC211823  PMID: 3027040

Abstract

Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to the existence of the two genes, called finO and finP; constitutive transfer of F is believed to be due to a lack of finO in F. In this paper, we report the identification and DNA sequence of the finO gene of R100, encoding a protein of 21,265 daltons. We show that F does actually encode finO, but the gene has been inactivated by insertion of IS3. Lederberg and Tatum (Nature [London] 158:558, 1946), who discovered sexuality in bacteria, may have had an Escherichia coli K-12 strain harboring such an finO F factor, which facilitated the generation of recombinant progeny useful for genetic analysis of bacteria and established the foundation for molecular genetics.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheah K. C., Ray A., Skurray R. Cloning and molecular analysis of the finO region from the antibiotic-resistance plasmid R6-5. Plasmid. 1984 Nov;12(3):222–226. doi: 10.1016/0147-619x(84)90050-7. [DOI] [PubMed] [Google Scholar]
  2. Dempsey W. B., McIntire S. A. The finO gene of antibiotic resistance plasmid R100. Mol Gen Genet. 1983;190(3):444–451. doi: 10.1007/BF00331075. [DOI] [PubMed] [Google Scholar]
  3. Finnegan D., Willetts N. The site of action of the F transfer inhibitor. Mol Gen Genet. 1973 Dec 31;127(4):307–316. doi: 10.1007/BF00267101. [DOI] [PubMed] [Google Scholar]
  4. Gaffney D., Skurray R., Willetts N. Regulation of the F conjugation genes studied by hybridization and tra-lacZ fusion. J Mol Biol. 1983 Jul 25;168(1):103–122. doi: 10.1016/s0022-2836(83)80325-8. [DOI] [PubMed] [Google Scholar]
  5. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  6. Hu S., Otsubo E., Davidson N., Saedler H. Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. J Bacteriol. 1975 May;122(2):764–775. doi: 10.1128/jb.122.2.764-775.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Machida Y., Machida C., Ohtsubo E. A novel type of transposon generated by insertion element IS102 present in a pSC101 derivative. Cell. 1982 Aug;30(1):29–36. doi: 10.1016/0092-8674(82)90008-3. [DOI] [PubMed] [Google Scholar]
  8. Manning P. A., Morelli G. DNA homology of the promoter-distal regions of the tra operons of sex factors F and R100 in Escherichia coli K-12. J Bacteriol. 1982 Apr;150(1):389–394. doi: 10.1128/jb.150.1.389-394.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  10. Meynell E., Meynell G. G., Datta N. Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriol Rev. 1968 Mar;32(1):55–83. doi: 10.1128/br.32.1.55-83.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mickel S., Ohtsubo E., Bauer W. Heteroduplex mapping of small plasmids derived from R-factor R12: in vivo recombination occurs at IS1 insertion sequences. Gene. 1977;2(3-4):193–210. doi: 10.1016/0378-1119(77)90017-8. [DOI] [PubMed] [Google Scholar]
  12. Miki T., Horiuchi T., Willetts N. S. Identification and characterization of four new tra cistrons on the E. coli K12 sex factor F. Plasmid. 1978 Jun;1(3):316–323. doi: 10.1016/0147-619x(78)90048-3. [DOI] [PubMed] [Google Scholar]
  13. Ohtsubo E., Rosenbloom M., Schrempf H., Goebel W., Rosen J. Site specific recombination involved in the generation of small plasmids. Mol Gen Genet. 1978 Feb 16;159(2):131–141. doi: 10.1007/BF00270886. [DOI] [PubMed] [Google Scholar]
  14. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sharp P. A., Cohen S. N., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol. 1973 Apr 5;75(2):235–255. doi: 10.1016/0022-2836(73)90018-1. [DOI] [PubMed] [Google Scholar]
  17. Sommer H., Cullum J., Saedler H. Integration of IS3 into IS2 generates a short sequence duplication. Mol Gen Genet. 1979;177(1):85–89. doi: 10.1007/BF00267256. [DOI] [PubMed] [Google Scholar]
  18. Taylor D. P., Greenberg J., Rownd R. H. Generation of miniplasmids from copy number mutants of the R plasmid NR1. J Bacteriol. 1977 Dec;132(3):986–995. doi: 10.1128/jb.132.3.986-995.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Timmerman K. P., Tu C. P. Complete sequence of IS3. Nucleic Acids Res. 1985 Mar 25;13(6):2127–2139. doi: 10.1093/nar/13.6.2127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Timmis K. N., Andrés I., Achtman M. Fertility repression of F-like conjugative plasmids: physical mapping of the R6--5 finO and finP cistrons and identification of the finO protein. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5836–5840. doi: 10.1073/pnas.75.12.5836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Willetts N., Skurray R. The conjugation system of F-like plasmids. Annu Rev Genet. 1980;14:41–76. doi: 10.1146/annurev.ge.14.120180.000353. [DOI] [PubMed] [Google Scholar]
  22. Willetts N., Wilkins B. Processing of plasmid DNA during bacterial conjugation. Microbiol Rev. 1984 Mar;48(1):24–41. doi: 10.1128/mr.48.1.24-41.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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