Abstract
Two research groups showed that several Bdellovibrio strains incorporated into their outer membranes intact OmpF porin proteins derived from their Escherichia coli prey. These results could not be reproduced by another group using Bdellovibrio bacteriovorus 109J. They showed that a major protein appearing in the Bdellovibrio Triton X-100-insoluble outer membrane was coded for by the bdellovibrios. We reconciled these results by examining the strain used by this group and by reviving a freeze-dried culture of strain 109J which had been stored for almost 9 years. B. bacteriovorus 109J failed to acquire substantial amounts of the OmpF protein from E. coli ML35, and a protein coded for by the bdellovibrios was expressed in its place. However, B. bacteriovorus 109J incorporated the OmpF protein from rough K-12 strains of E. coli, and the revived 9-year-old culture of B. bacteriovorus 109J incorporated more of the OmpF protein from the smooth E. coli ML35 than did its contemporary counterpart. The protein isolated from the outer membrane of the bdellovibrios was identified as the OmpF protein of E. coli by its protease peptide profile on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by Western blot analysis. This confirmed that bdellovibrios relocalize outer membrane proteins from their prey, but relocalization may be an unstable trait which can be influenced by the prey.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984 Jan;136(1):175–179. doi: 10.1016/0003-2697(84)90320-8. [DOI] [PubMed] [Google Scholar]
- Blasband A. J., Marcotte W. R., Jr, Schnaitman C. A. Structure of the lc and nmpC outer membrane porin protein genes of lambdoid bacteriophage. J Biol Chem. 1986 Sep 25;261(27):12723–12732. [PubMed] [Google Scholar]
- Diedrich D. L., Duran C. P., Conti S. F. Acquisition of Escherichia coli outer membrane proteins by Bdellovibrio sp. strain 109D. J Bacteriol. 1984 Jul;159(1):329–334. doi: 10.1128/jb.159.1.329-334.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
- Guerrini F., Romano V., Valenzi M., Di Giulio M., Mupo M. R., Sacco M. Molecular parasitism in the Escherichia coli-Bdellovibrio bacteriovorus system: translocation of the matrix protein from the host to the parasite outer membrane. EMBO J. 1982;1(11):1439–1444. doi: 10.1002/j.1460-2075.1982.tb01335.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuenen J. G., Rittenberg S. C. Incorporation of long-chain fatty acids of the substrate organism by Bdellovibrio bacteriovorus during intraperiplasmic growth. J Bacteriol. 1975 Mar;121(3):1145–1157. doi: 10.1128/jb.121.3.1145-1157.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Nelson D. R., Rittenberg S. C. Incorporation of substrate cell lipid A components into the lipopolysaccharide of intraperiplasmically grown Bdellovibrio bacteriovorus. J Bacteriol. 1981 Sep;147(3):860–868. doi: 10.1128/jb.147.3.860-868.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rayner J. R., Cover W. H., Martinez R. J., Rittenberg S. C. Bdellovibrio bacteriovorus synthesizes an OmpF-like outer membrane protein during both axenic and intraperiplasmic growth. J Bacteriol. 1985 Aug;163(2):595–599. doi: 10.1128/jb.163.2.595-599.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rittenberg S. C. Nonidentity of Bdellovibrio bacteriovorus strains 109D and 109J. J Bacteriol. 1972 Jan;109(1):432–433. doi: 10.1128/jb.109.1.432-433.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruby E. G., McCabe J. B. An ATP transport system in the intracellular bacterium, Bdellovibrio bacteriovorus 109J. J Bacteriol. 1986 Sep;167(3):1066–1070. doi: 10.1128/jb.167.3.1066-1070.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STOLP H., STARR M. P. BDELLOVIBRIO BACTERIOVORUS GEN. ET SP. N., A PREDATORY, ECTOPARASITIC, AND BACTERIOLYTIC MICROORGANISM. Antonie Van Leeuwenhoek. 1963;29:217–248. doi: 10.1007/BF02046064. [DOI] [PubMed] [Google Scholar]
- Starr M. P., Huang J. C. Physiology of the bdellovibrios. Adv Microb Physiol. 1972;8:215–261. doi: 10.1016/s0065-2911(08)60191-5. [DOI] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]