Abstract
Recent studies indicate that both CD4+ and CD8+ T lymphocytes proliferate in vitro in response to Mls-1a-encoded determinants. Using both immunogenetic and antibody blocking approaches we show here that Mls-1a responses of both subsets require expression of major histocompatibility complex (MHC) class II molecules (I-A and/or I-E) by the stimulator cells. Furthermore, CD8+ T cell responses to Mls- 1a/class II MHC do not require (and are in fact inhibited by) the presence of functional CD8 molecules. Taken together, our data underscore the dramatic differences between CD8+ T cell responses to conventional peptide antigens as opposed to "superantigens" such as Mls- 1a.
Full Text
The Full Text of this article is available as a PDF (321.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bierer B. E., Sleckman B. P., Ratnofsky S. E., Burakoff S. J. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989;7:579–599. doi: 10.1146/annurev.iy.07.040189.003051. [DOI] [PubMed] [Google Scholar]
- Festenstein H., Berumen L. BALB.D2-Mlsa--a new congenic mouse strain. Transplantation. 1984 Mar;37(3):322–324. doi: 10.1097/00007890-198403000-00024. [DOI] [PubMed] [Google Scholar]
- Festenstein H. Immunogenetic and biological aspects of in vitro lymphocyte allotransformation (MLR) in the mouse. Transplant Rev. 1973;15:62–88. doi: 10.1111/j.1600-065x.1973.tb00111.x. [DOI] [PubMed] [Google Scholar]
- Happ M. P., Woodland D. L., Palmer E. A third T-cell receptor beta-chain variable region gene encodes reactivity to Mls-1a gene products. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6293–6296. doi: 10.1073/pnas.86.16.6293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herrmann T., Maryanski J. L., Romero P., Fleischer B., MacDonald H. R. Activation of MHC class I-restricted CD8+ CTL by microbial T cell mitogens. Dependence upon MHC class II expression of the target cells and V beta usage of the responder T cells. J Immunol. 1990 Feb 15;144(4):1181–1186. [PubMed] [Google Scholar]
- Janeway C. A., Jr, Yagi J., Conrad P. J., Katz M. E., Jones B., Vroegop S., Buxser S. T-cell responses to Mls and to bacterial proteins that mimic its behavior. Immunol Rev. 1989 Feb;107:61–88. doi: 10.1111/j.1600-065x.1989.tb00003.x. [DOI] [PubMed] [Google Scholar]
- Kanagawa O., Maki R. Inhibition of MHC class II-restricted T cell response by Lyt-2 alloantigen. J Exp Med. 1989 Sep 1;170(3):901–912. doi: 10.1084/jem.170.3.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Larsson-Sciard E. L., Spetz-Hagberg A. L., Casrouge A., Kourilsky P. Analysis of T cell receptor V beta gene usage in primary mixed lymphocyte reactions: evidence for directive usage by different antigen-presenting cells and Mls-like determinants on T cell blasts. Eur J Immunol. 1990 Jun;20(6):1223–1229. doi: 10.1002/eji.1830200605. [DOI] [PubMed] [Google Scholar]
- MacDonald H. R., Lees R. K., Chvatchko Y. CD8+ T cells respond clonally to Mls-1a-encoded determinants. J Exp Med. 1990 Apr 1;171(4):1381–1386. doi: 10.1084/jem.171.4.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swain S. L. T cell subsets and the recognition of MHC class. Immunol Rev. 1983;74:129–142. doi: 10.1111/j.1600-065x.1983.tb01087.x. [DOI] [PubMed] [Google Scholar]
- Webb S. R., Sprent J. Response of mature unprimed CD8+ T cells to Mlsa determinants. J Exp Med. 1990 Mar 1;171(3):953–958. doi: 10.1084/jem.171.3.953. [DOI] [PMC free article] [PubMed] [Google Scholar]