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. 1987 Mar;169(3):1217–1222. doi: 10.1128/jb.169.3.1217-1222.1987

Activities of the RNAI and RNAII promoters of plasmid pBR322.

S Lin-Chao, H Bremer
PMCID: PMC211922  PMID: 2434459

Abstract

The synthesis rates of the replication control RNAs of plasmid pBR322, RNAI, an inhibitor of replication, and RNAII, the preprimer, have been determined by hybridizing in vivo pulse-labeled RNA to specific, single-stranded DNA probes for RNAI and RNAII. In Escherichia coli growing in glycerol minimal medium, RNAI transcripts were made at a rate of one molecule per 30 s per plasmid; RNAII was transcribed fivefold less, at a rate of one molecule per 3 min per plasmid. It is estimated that only 1 in 20 prepriming events leads to replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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