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. 1987 Apr;169(4):1386–1390. doi: 10.1128/jb.169.4.1386-1390.1987

Conditions leading to secretion of a normally periplasmic protein in Escherichia coli.

J M Pages, J Anba, C Lazdunski
PMCID: PMC211957  PMID: 3549684

Abstract

The phosphate-binding protein (PhoS) is a periplasmic protein which is part of the high-affinity phosphate transport system of Escherichia coli. Hyperproduction of PhoS in strains carrying a multicopy plasmid containing phoS led to partial secretion of the protein. By 6 h after transfer to phosphate-limiting medium, about 13% of the total newly synthesized PhoS was secreted to the medium. Kinetic studies demonstrated that this secretion consists of newly synthesized PhoS. This secretion occurs in PhoS-hyperproducer strains but not in a PhoS-overproducer strain. Another type of secretion concerning periplasmic PhoS was observed in both PhoS-hyperproducer and PhoS-overproducer strains. This mode of secretion depended upon the addition of phosphate to cells previously grown in phosphate-limiting medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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