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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Apr;169(4):1763–1766. doi: 10.1128/jb.169.4.1763-1766.1987

Nucleotide sequence and expression of the beta-lactamase gene from Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus subtilis, and Staphylococcus aureus.

P Z Wang, R P Novick
PMCID: PMC212015  PMID: 3104315

Abstract

The structural gene for beta-lactamase in the Staphylococcus aureus plasmid pI258 was cloned into a Staphylococcus aureus-Bacillus subtilis-Escherichia coli shuttle vector, pWN101, and the nucleotide sequence of the gene was determined. pWN101 was structurally stable and the beta-lactamase gene was expressed efficiently from its native promoter and ribosome-binding site in all three hosts.

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Selected References

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