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. 1987 Apr;169(4):1767–1771. doi: 10.1128/jb.169.4.1767-1771.1987

Conditional transduction of Salmonella typhimurium envB mutations.

D N Antón
PMCID: PMC212016  PMID: 3549704

Abstract

Joint transduction of the argR and envB genes was observed, at a frequency of 24.5%, when four envB strains were transduced to tetracycline resistance (Tetr) with bacteriophage P22 grown on an argR372::Tn10 envB+ donor. When round-cell argR372::Tn10 derivatives of those envB strains were used as donors, two of them did not produce envB transductants in wild-type LT2 and other envB+ recipients, even though large numbers of Tetr transductants were obtained. This apparent exclusion of envB mutations did not occur when mecillinam-resistant derivatives of those envB+ strains were used as recipients. Mutations conferring partial resistance to mecillinam were found, unlinked to the argR-envB region, in three of the four envB strains studied; envB+ derivatives of the four strains were competent to accept envB mutations excluded by wild-type recipients. It is suggested that some envB mutations are lethal in the absence of suppressor mutations, some of which increase resistance to mecillinam.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Terry C. E., Hardigree A. A. Giant cells of Escherichia coli. J Bacteriol. 1968 Jan;95(1):139–142. doi: 10.1128/jb.95.1.139-142.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson R. P., Roth J. R. Tandem chromosomal duplications in Salmonella typhimurium: fusion of histidine genes to novel promoters. J Mol Biol. 1978 Feb 15;119(1):147–166. doi: 10.1016/0022-2836(78)90274-7. [DOI] [PubMed] [Google Scholar]
  3. Antón D. N., Orce L. V. Envelope mutation promoting autolysis in Salmonella typhimurium. Mol Gen Genet. 1976 Feb 27;144(1):97–105. doi: 10.1007/BF00277311. [DOI] [PubMed] [Google Scholar]
  4. Antón D. N., de Micheli A. T., Palermo A. M. Isolation of round-cell mutants of Salmonella typhimurium. Can J Microbiol. 1983 Feb;29(2):170–173. doi: 10.1139/m83-029. [DOI] [PubMed] [Google Scholar]
  5. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gardner M. M., Hennig D. O., Kelln R. A. Control of arg gene expression in Salmonella typhimurium by the arginine repressor from Escherichia coli K-12. Mol Gen Genet. 1983;189(3):458–462. doi: 10.1007/BF00325909. [DOI] [PubMed] [Google Scholar]
  7. Iwaya M., Jones C. W., Khorana J., Strominger J. L. Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli. J Bacteriol. 1978 Jan;133(1):196–202. doi: 10.1128/jb.133.1.196-202.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kelln R. A., Foltermann K. F., O'Donovan G. A. Location of the argR gene on the chromosome of Salmonella typhimurium. Mol Gen Genet. 1975 Sep 8;139(4):277–284. doi: 10.1007/BF00267967. [DOI] [PubMed] [Google Scholar]
  9. Matsuhashi S., Kamiryo T., Blumberg P. M., Linnett P., Willoughby E., Strominger J. L. Mechanism of action and development of resistance to a new amidino penicillin. J Bacteriol. 1974 Feb;117(2):578–587. doi: 10.1128/jb.117.2.578-587.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matsuzawa H., Hayakawa K., Sato T., Imahori K. Characterization and genetic analysis of a mutant of Escherichia coli K-12 with rounded morphology. J Bacteriol. 1973 Jul;115(1):436–442. doi: 10.1128/jb.115.1.436-442.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Normark S. Mutation in Escherichia coli K-12 mediating spherelike envelopes and changes tolerance to ultraviolet irradiation and some antibiotics. J Bacteriol. 1969 Jun;98(3):1274–1277. doi: 10.1128/jb.98.3.1274-1277.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sompolinsky D., Samra Z. Influence of magnesium and manganese on some biological and physical properties of tetracycline. J Bacteriol. 1972 May;110(2):468–476. doi: 10.1128/jb.110.2.468-476.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sonntag I., Schwarz H., Hirota Y., Henning U. Cell envelope and shape of Escherichia coli: multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins. J Bacteriol. 1978 Oct;136(1):280–285. doi: 10.1128/jb.136.1.280-285.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spratt B. G., Pardee A. B. Penicillin-binding proteins and cell shape in E. coli. Nature. 1975 Apr 10;254(5500):516–517. doi: 10.1038/254516a0. [DOI] [PubMed] [Google Scholar]
  15. Tamaki S., Matsuzawa H., Matsuhashi M. Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli. J Bacteriol. 1980 Jan;141(1):52–57. doi: 10.1128/jb.141.1.52-57.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Westling-Häggström B., Normark S. Genetic and physiological analysis of an envB spherelike mutant of Escherichia coli K-12 and characterization of its transductants. J Bacteriol. 1975 Jul;123(1):75–82. doi: 10.1128/jb.123.1.75-82.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wyche J. H., Kennedy J., Hartman Z., Hartman P. E., Diven J. Round-cell mutant of Salmonella typhimurium. J Bacteriol. 1974 Nov;120(2):965–969. doi: 10.1128/jb.120.2.965-969.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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