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. 1987 May;169(5):1824–1829. doi: 10.1128/jb.169.5.1824-1829.1987

Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis.

G W Owttrim, J R Coleman
PMCID: PMC212031  PMID: 3032896

Abstract

A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as recombination proficiency when measured by Hfr-mediated conjugation. The cyanobacterial recA gene restored spontaneous but not mitomycin C-induced prophage production. Restriction analysis and subcloning yielded a 1.5-kilobase-pair Sau3A fragment which also restored methylmethane sulfonate resistance and coded for a 38- to 40-kilodalton polypeptide when expressed in an in vitro transcription-translation system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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