Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 May;169(5):1960–1966. doi: 10.1128/jb.169.5.1960-1966.1987

Effect of growth temperature on the lipids, outer membrane proteins, and lipopolysaccharides of Pseudomonas aeruginosa PAO.

A M Kropinski, V Lewis, D Berry
PMCID: PMC212060  PMID: 3106325

Abstract

Growth of Pseudomonas aeruginosa PAO1 at 15 to 45 degrees C in tryptic soy broth resulted in changes in the lipids, lipopolysaccharides (LPSs), and outer membrane proteins of the cells. Cells grown at 15 degrees C contained, relative to those cultivated at 45 degrees C, increased levels of the phospholipid fatty acids hexadecenoate and octadecenoate and reduced levels of the corresponding saturated fatty acids. Furthermore, the lipid A fatty acids also showed thermoadaptation with decreases in dodecanoic and hexadecanoic acids and increases in the level of 3-hydroxydecanoate and 2-hydroxdodecanoate as the growth temperature decreased. In addition, LPS extracted from cells cultivated at the lower temperatures contained a higher content of long-chain S-form molecules than that isolated from cells grown at higher temperatures. On the other hand, the percentage of LPS cores substituted with side-chain material decreased from 37.6 mol% at 45 degrees C to 19.3 mol% at 15 degrees C. The outer membrane protein profiles indicated that at low growth temperatures there was an increase in a polypeptide with an apparent molecular weight of 43,000 and decreases in the content of 21,000 (protein H1)- and 27,500-molecular-weight proteins.

Full text

PDF
1960

Images in this article

1962Image
on p.1962

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acker G., Wartenberg K., Knapp W. Zuckerzusammensetzung des Lipopolysaccharids und Feinstruktur der äusseren Membran (Zellwand) bei Yersinia enterocolitica. Zentralbl Bakteriol A. 1980;247(2):229–240. [PubMed] [Google Scholar]
  2. Angus B. L., Carey A. M., Caron D. A., Kropinski A. M., Hancock R. E. Outer membrane permeability in Pseudomonas aeruginosa: comparison of a wild-type with an antibiotic-supersusceptible mutant. Antimicrob Agents Chemother. 1982 Feb;21(2):299–309. doi: 10.1128/aac.21.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhakoo M., Herbert R. A. Fatty acid and phospholipid composition of five psychrotrophic Pseudomonas spp. grown at different temperatures. Arch Microbiol. 1980 May;126(1):51–55. doi: 10.1007/BF00421890. [DOI] [PubMed] [Google Scholar]
  4. Cadieux J. E., Kuzio J., Milazzo F. H., Kropinski A. M. Spontaneous release of lipopolysaccharide by Pseudomonas aeruginosa. J Bacteriol. 1983 Aug;155(2):817–825. doi: 10.1128/jb.155.2.817-825.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chester I. R., Meadow P. M. Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa. Eur J Biochem. 1975 Oct 15;58(2):273–282. doi: 10.1111/j.1432-1033.1975.tb02373.x. [DOI] [PubMed] [Google Scholar]
  6. Darveau R. P., Charnetzky W. T., Hurlbert R. F., Hancock R. E. Effects of growth temperature, 47-megadalton plasmid, and calcium deficiency on the outer membrane protein porin and lipopolysaccharide composition of Yersinia pestis EV76. Infect Immun. 1983 Dec;42(3):1092–1101. doi: 10.1128/iai.42.3.1092-1101.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Darveau R. P., Hancock R. E. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol. 1983 Aug;155(2):831–838. doi: 10.1128/jb.155.2.831-838.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dmitriev B. A., Knirel Y. A., Kocharova N. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. Somatic antigens of Pseudomonas aeruginosa. The structure of the polysaccharide chain of Ps. aeruginosa O-serogroup 7 (Lanyi) lipopolysacharide. Eur J Biochem. 1980 May;106(2):643–651. doi: 10.1111/j.1432-1033.1980.tb04612.x. [DOI] [PubMed] [Google Scholar]
  9. Drewry D. T., Lomax J. A., Gray G. W., Wilkinson S. G. Studies of lipid A fractions from the lipopolysaccharides of Pseudomonas aeruginosa and Pseudomonas alcaligenes. Biochem J. 1973 Jul;133(3):563–572. doi: 10.1042/bj1330563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gill C. O. Effect of growth temperature on the lipids of Pseudomonas fluorescens. J Gen Microbiol. 1975 Aug;89(2):293–298. doi: 10.1099/00221287-89-2-293. [DOI] [PubMed] [Google Scholar]
  11. Gill C. O., Suisted J. R. The effects of temperature and growth rate on the proportion of unsaturated fatty acids in bacterial lipids. J Gen Microbiol. 1978 Jan;104(1):31–36. doi: 10.1099/00221287-104-1-31. [DOI] [PubMed] [Google Scholar]
  12. Hancock I. C., Meadow P. M. The extractable lipids of Pseudomonas aeruginosa. Biochim Biophys Acta. 1969 Oct 28;187(3):366–379. doi: 10.1016/0005-2760(69)90010-1. [DOI] [PubMed] [Google Scholar]
  13. Hancock R. E., Carey A. M. Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins. J Bacteriol. 1979 Dec;140(3):902–910. doi: 10.1128/jb.140.3.902-910.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hancock R. E., Mutharia L. M., Chan L., Darveau R. P., Speert D. P., Pier G. B. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains. Infect Immun. 1983 Oct;42(1):170–177. doi: 10.1128/iai.42.1.170-177.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jarrell K. F., Kropinski A. M. Isolation and characterization of a bacteriophage specific for the lipopolysaccharide of rough derivatives of Pseudomonas aeruginosa strain PAO. J Virol. 1981 May;38(2):529–538. doi: 10.1128/jvi.38.2.529-538.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Johnson A. R. Improved method of hexosamine determination. Anal Biochem. 1971 Dec;44(2):628–635. doi: 10.1016/0003-2697(71)90252-1. [DOI] [PubMed] [Google Scholar]
  18. Johnston N. C., Goldfine H. Effects of growth temperature on fatty acid and alk-1-enyl group compositions of Veillonella parvula and Megasphaera elsdenii phospholipids. J Bacteriol. 1982 Feb;149(2):567–575. doi: 10.1128/jb.149.2.567-575.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. KATES M., HAGEN P. O. INFLUENCE OF TEMPERATURE ON FATTY ACID COMPOSITION OF PSYCHROPHILIC AND MESOPHILIC SERRATIA SPECIES. Can J Biochem. 1964 Apr;42:481–488. doi: 10.1139/o64-055. [DOI] [PubMed] [Google Scholar]
  20. Kropinski A. M., Jewell B., Kuzio J., Milazzo F., Berry D. Structure and functions of Pseudomonas aeruginosa lipopolysaccharide. Antibiot Chemother (1971) 1985;36:58–73. doi: 10.1159/000410472. [DOI] [PubMed] [Google Scholar]
  21. Kropinski A. M., Kuzio J., Angus B. L., Hancock R. E. Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1982 Feb;21(2):310–319. doi: 10.1128/aac.21.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  23. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. McConnell M., Wright A. Variation in the structure and bacteriophage-inactivating capacity of Salmonella anatum lipopolysaccharide as a function of growth temperature. J Bacteriol. 1979 Feb;137(2):746–751. doi: 10.1128/jb.137.2.746-751.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. McDonald I. J., Adams G. A. Influence of cultural conditions on the lipopolysaccharide composition of Neisseria sicca. J Gen Microbiol. 1971 Feb;65(2):201–207. doi: 10.1099/00221287-65-2-201. [DOI] [PubMed] [Google Scholar]
  26. McElhaney R. N., Souza K. A. The relationship between environmental temperature, cell growth and the fluidity and physical state of the membrane lipids in Bacillus stearothermophilus. Biochim Biophys Acta. 1976 Sep 7;443(3):348–359. doi: 10.1016/0005-2736(76)90455-7. [DOI] [PubMed] [Google Scholar]
  27. Miguez C. B., Beveridge T. J., Ingram J. M. Lipopolysaccharide changes and cytoplasmic polyphosphate granule accumulation in Pseudomonas aeruginosa during growth on hexadecane. Can J Microbiol. 1986 Mar;32(3):248–253. doi: 10.1139/m86-049. [DOI] [PubMed] [Google Scholar]
  28. Miller K. J. Effects of temperature and sodium chloride concentration on the phospholipid and fatty acid compositions of a halotolerant Planococcus sp. J Bacteriol. 1985 Apr;162(1):263–270. doi: 10.1128/jb.162.1.263-270.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Moss C. W., Dees S. B. Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens. J Clin Microbiol. 1976 Dec;4(6):492–502. doi: 10.1128/jcm.4.6.492-502.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Moss C. W., Samuels S. B., Weaver R. E. Cellular fatty acid composition of selected Pseudomonas species. Appl Microbiol. 1972 Oct;24(4):596–598. doi: 10.1128/am.24.4.596-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Nakae T. Outer-membrane permeability of bacteria. Crit Rev Microbiol. 1986;13(1):1–62. doi: 10.3109/10408418609108734. [DOI] [PubMed] [Google Scholar]
  32. Rottem S., Markowitz O., Razin S. Thermal regulation of the fatty acid composition of lipopolysaccharides and phospholipids of Proteus mirabilis. Eur J Biochem. 1978 Apr 17;85(2):445–450. doi: 10.1111/j.1432-1033.1978.tb12258.x. [DOI] [PubMed] [Google Scholar]
  33. Smyth C. J., Jonsson P., Olsson E., Soderlind O., Rosengren J., Hjertén S., Wadström T. Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography. Infect Immun. 1978 Nov;22(2):462–472. doi: 10.1128/iai.22.2.462-472.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  35. Van Alphen L., Lugtenberg B., Rietschel E. T., Mombers C. Architecture of the outer membrane of Escherichia coli K12. Phase transitions of the bacteriophage K3 receptor complex. Eur J Biochem. 1979 Nov;101(2):571–579. doi: 10.1111/j.1432-1033.1979.tb19752.x. [DOI] [PubMed] [Google Scholar]
  36. Wartenberg K., Knapp W., Ahamed N. M., Widemann C., Mayer H. Temperature-dependent changes in the sugar and fatty acid composition of lipopolysaccharides from Yersinia enterocolitica strains. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Feb;253(4):523–530. [PubMed] [Google Scholar]
  37. Wilkinson S. G. Composition and structure of lipopolysaccharides from Pseudomonas aeruginosa. Rev Infect Dis. 1983 Nov-Dec;5 (Suppl 5):S941–S949. doi: 10.1093/clinids/5.supplement_5.s941. [DOI] [PubMed] [Google Scholar]
  38. Wollenweber H. W., Schlecht S., Lüderitz O., Rietschel E. T. Fatty acid in lipopolysaccharides of Salmonella species grown at low temperature. Identification and position. Eur J Biochem. 1983 Jan 17;130(1):167–171. doi: 10.1111/j.1432-1033.1983.tb07132.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES