Abstract
The activities of several oxidoreductases were measured in three fermentative and two nonfermentative Mycoplasma species that were grown under aerobic or anaerobic conditions. Acholeplasma laidlawii MG, Mycoplasma hyorhinis GDL, and Mycoplasma pneumoniae FH had very high apparent activities of pyruvate dehydrogenase and pyruvate dehydrogenase complex compared with the activities of mammalian fibroblasts or human platelet-enriched preparations, while Mycoplasma salivarium VV and Mycoplasma arthritidis 07 had very low apparent activities of these two enzymes. Strictly anaerobic growth diminished both enzymatic activities. The activity of alpha-ketoglutarate dehydrogenase complex was minimal in all five mycoplasmas that were grown under aerobic conditions, anaerobic conditions, or both. All the mycoplasmas that were examined exhibited lactate dehydrogenase and NADH-dichlorophenol indophenol oxidoreductase activities. The properties of mycoplasmal pyruvate dehydrogenase complex suggest that it differs from the mammalian enzyme.
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Selected References
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- Barile M. F., Schimke R. T., Riggs D. B. Presence of the arginine dihydrolase pathway in Mycoplasma. J Bacteriol. 1966 Jan;91(1):189–192. doi: 10.1128/jb.91.1.189-192.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blass J. P., Cederbaum S. D., Kark R. A. Rapid diagnosis of pyruvate and ketoglutarate dehydrogenase deficiencies in platelet-enriched preparations from blood. Clin Chim Acta. 1977 Feb 15;75(1):21–30. doi: 10.1016/0009-8981(77)90496-x. [DOI] [PubMed] [Google Scholar]
- CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
- Kresze G. B. An improved procedure for the assay of pyruvate dehydrogenase. Anal Biochem. 1979 Sep 15;98(1):85–88. doi: 10.1016/0003-2697(79)90709-7. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Muhlrad A., Peleg I., Robertson J. A., Robinson I. M., Kahane I. Acetate kinase activity in mycoplasmas. J Bacteriol. 1981 Jul;147(1):271–273. doi: 10.1128/jb.147.1.271-273.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RODWELL A. W., RODWELL E. S. The breakdown of pyruvate by Asterococcus mycoides, the organism of bovine pleuropneumonia. Aust J Biol Sci. 1954 Feb;7(1):31–36. [PubMed] [Google Scholar]
- Sakurai Y., Fekuyoshi Y., Hamada M., Hayakawa T., Koike M. Mammalian alpha-keto acid dehydrogenase complexes. VI. Nature of the multiple forms of pig heart lipoamide dehydrogenase. J Biol Chem. 1970 Sep 10;245(17):4453–4462. [PubMed] [Google Scholar]
- Schimke R. T., Berlin C. M., Sweeney E. W., Carroll W. R. The generation of energy by the arginine dihydrolase pathway in Mycoplasma hominis 07. J Biol Chem. 1966 May 25;241(10):2228–2236. [PubMed] [Google Scholar]
- VANDEMARK P. J., SMITH P. F. EVIDENCE FOR A TRICARBOXYLIC ACID CYCLE IN MYCOPLASMA HOMINIS. J Bacteriol. 1964 Dec;88:1602–1607. doi: 10.1128/jb.88.6.1602-1607.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]