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. 1987 May;169(5):2150–2157. doi: 10.1128/jb.169.5.2150-2157.1987

Physiological adaptations of anaerobic bacteria to low pH: metabolic control of proton motive force in Sarcina ventriculi.

S Goodwin, J G Zeikus
PMCID: PMC212116  PMID: 3571164

Abstract

Detailed physiological studies were done to compare the influence of environmental pH and fermentation end product formation on metabolism, growth, and proton motive force in Sarcina ventriculi. The kinetics of end product formation during glucose fermentation in unbuffered batch cultures shifted from hydrogen-acetate production to ethanol production as the medium pH dropped from 7.0 to 3.3. At a constant pH of 3.0, the production of acetate ceased when the accumulation of acetate in the medium reached 40 mmol/liter. At a constant pH of 7.0, acetate production continued throughout the entire growth time course. The in vivo hydrogenase activity was much higher in cells grown at pH 7.0 than at pH 3.0. The magnitude of the proton motive force increased in relation to a decrease of the medium pH from 7.5 to 3.0. When the organism was grown at pH 3.0, the cytoplasmic pH was 4.25 and the organism was unable to exclude acetic acid or butyric acid from the cytoplasm. Addition of acetic acid, but not hydrogen or ethanol, inhibited growth and resulted in proton motive force dissipation and the accumulation of acetic acid in the cytoplasm. The results indicate that S. ventriculi is an acidophile that can continue to produce ethanol at low cytoplasmic pH values. Both the ability to shift to ethanol production and the ability to continue to ferment glucose while cytoplasmic pH values are low adapt S. ventriculi for growth at low pH.

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Selected References

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  1. BAUCHOP T., DAWES E. A. Metabolism of pyruvic and formic acids by Zymosarcina ventriculi. Biochim Biophys Acta. 1959 Nov;36:294–296. doi: 10.1016/0006-3002(59)90114-3. [DOI] [PubMed] [Google Scholar]
  2. Baronofsky J. J., Schreurs W. J., Kashket E. R. Uncoupling by Acetic Acid Limits Growth of and Acetogenesis by Clostridium thermoaceticum. Appl Environ Microbiol. 1984 Dec;48(6):1134–1139. doi: 10.1128/aem.48.6.1134-1139.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Canale-Parola E. Biology of the sugar-fermenting Sarcinae. Bacteriol Rev. 1970 Mar;34(1):82–97. doi: 10.1128/br.34.1.82-97.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goodwin S., Zeikus J. G. Ecophysiological adaptations of anaerobic bacteria to low pH: analysis of anaerobic digestion in acidic bog sediments. Appl Environ Microbiol. 1987 Jan;53(1):57–64. doi: 10.1128/aem.53.1.57-64.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hsung J. C., Haug A. Intracellular pH of Thermoplasma acidophila. Biochim Biophys Acta. 1975 May 21;389(3):477–482. doi: 10.1016/0005-2736(75)90158-3. [DOI] [PubMed] [Google Scholar]
  7. Hsung J. C., Haug A. Membrane potential of Thermoplasma acidophila. FEBS Lett. 1977 Jan 15;73(1):47–50. doi: 10.1016/0014-5793(77)80011-2. [DOI] [PubMed] [Google Scholar]
  8. Huang L., Gibbins L. N., Forsberg C. W. Transmembrane pH gradient and membrane potential in Clostridium acetobutylicum during growth under acetogenic and solventogenic conditions. Appl Environ Microbiol. 1985 Oct;50(4):1043–1047. doi: 10.1128/aem.50.4.1043-1047.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kashket E. R., Barker S. L. Effects of potassium ions on the electrical and pH gradients across the membrane of Streptococcus lactis cells. J Bacteriol. 1977 Jun;130(3):1017–1023. doi: 10.1128/jb.130.3.1017-1023.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kashket E. R., Blanchard A. G., Metzger W. C. Proton motive force during growth of Streptococcus lactis cells. J Bacteriol. 1980 Jul;143(1):128–134. doi: 10.1128/jb.143.1.128-134.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kashket E. R. Effects of aerobiosis and nitrogen source on the proton motive force in growing Escherichia coli and Klebsiella pneumoniae cells. J Bacteriol. 1981 Apr;146(1):377–384. doi: 10.1128/jb.146.1.377-384.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kashket E. R. Proton motive force in growing Streptococcus lactis and Staphylococcus aureus cells under aerobic and anaerobic conditions. J Bacteriol. 1981 Apr;146(1):369–376. doi: 10.1128/jb.146.1.369-376.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matin A., Wilson B., Zychlinsky E., Matin M. Proton motive force and the physiological basis of delta pH maintenance in thiobacillus acidophilus. J Bacteriol. 1982 May;150(2):582–591. doi: 10.1128/jb.150.2.582-591.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Riebeling V., Thauer R. K., Jungermann K. The internal-alkaline pH gradient, sensitive to uncoupler and ATPase inhibitor, in growing Clostridium pasteurianum. Eur J Biochem. 1975 Jul 1;55(2):445–453. doi: 10.1111/j.1432-1033.1975.tb02181.x. [DOI] [PubMed] [Google Scholar]
  15. Schink B., Lupton F. S., Zeikus J. G. Radioassay for hydrogenase activity in viable cells and documentation of aerobic hydrogen-consuming bacteria living in extreme environments. Appl Environ Microbiol. 1983 May;45(5):1491–1500. doi: 10.1128/aem.45.5.1491-1500.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schwartz R. D., Keller F. A. Acetic Acid Production by Clostridium thermoaceticum in pH-Controlled Batch Fermentations at Acidic pH. Appl Environ Microbiol. 1982 Jun;43(6):1385–1392. doi: 10.1128/aem.43.6.1385-1392.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stephenson M. P., Dawes E. A. Pyruvic acid and formic acid metabolism in Sarcina ventriculi and the role of ferredoxin. J Gen Microbiol. 1971 Dec;69(3):331–343. doi: 10.1099/00221287-69-3-331. [DOI] [PubMed] [Google Scholar]
  18. Thauer R. K., Jungermann K., Decker K. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev. 1977 Mar;41(1):100–180. doi: 10.1128/br.41.1.100-180.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zychlinsky E., Matin A. Effect of starvation on cytoplasmic pH, proton motive force, and viability of an acidophilic bacterium, Thiobacillus acidophilus. J Bacteriol. 1983 Jan;153(1):371–374. doi: 10.1128/jb.153.1.371-374.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. ten Brink B., Konings W. N. Electrochemical proton gradient and lactate concentration gradient in Streptococcus cremoris cells grown in batch culture. J Bacteriol. 1982 Nov;152(2):682–686. doi: 10.1128/jb.152.2.682-686.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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