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. 1987 Jun;169(6):2643–2649. doi: 10.1128/jb.169.6.2643-2649.1987

Isolation of Haemophilus influenzae genes that suppress Escherichia coli polA mutations.

G L Williams, B Seaton, D McCarthy
PMCID: PMC212144  PMID: 3294801

Abstract

Haemophilus influenzae was found to produce a DNA polymerase that was similar to polymerase I of Escherichia coli. E. coli polA mutants were used as backgrounds for the selection of H. influenzae polA suppressor genes. Six different H. influenzae fragments were isolated that could suppress E. coli polA mutations. None of the suppressors appeared to encode the H. influenzae equivalent of the E. coli polA gene. One type of clone, represented by pGW41, caused a polymerase I activity to appear in a suppressed polA1 mutant. Plasmids from the pGW41 class contained two genes (pol-2 and pol-3) that were both required for polA suppression. Mutated nonsuppressing derivatives of the pGW41 class were used to create H. influenzae mutants that were deficient in polymerase I.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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