Abstract
The genes ptsI and ptsH, which encode, respectively, enzyme I and Hpr, cytoplasmic proteins involved in the phosphoenolpyruvate:sugar phosphotransferase system, were cloned from Bacillus subtilis. A plasmid containing a 4.1-kilobase DNA fragment was shown to complement Escherichia coli mutations affecting the ptsH and ptsI genes. In minicells this plasmid expressed two proteins with the molecular weights expected for Hpr and enzyme I. Therefore, ptsH and ptsI are adjacent in B. subtilis, as in E. coli. In E. coli a third gene (crr), involved in glucose translocation and also in catabolite repression, is located downstream from the ptsHI operon. The 4.1-kilobase fragment from B. subtilis was shown to contain a gene that enables an E. coli crr mutant to use glucose. This gene, unlike the E. coli crr gene, was located to the left of ptsH.
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Selected References
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- De Reuse H., Huttner E., Danchin A. Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: evidence for the existence of a single transcriptional unit. Gene. 1984 Dec;32(1-2):31–40. doi: 10.1016/0378-1119(84)90029-5. [DOI] [PubMed] [Google Scholar]
- Ferrari F. A., Nguyen A., Lang D., Hoch J. A. Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol. 1983 Jun;154(3):1513–1515. doi: 10.1128/jb.154.3.1513-1515.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gay P., Cordier P., Marquet M., Delobbe A. Carbohydrate metabolism and transport in Bacillus subtilis. A study of ctr mutations. Mol Gen Genet. 1973 Mar 19;121(4):355–368. doi: 10.1007/BF00433234. [DOI] [PubMed] [Google Scholar]
- Marquet M., Creignou M. C., Dedonder R. The phosphoenolpyruvate : methyl-alpha-D-glucoside phosphotransferase system in Bacillus subtilis Marburg 168 : purification and identification of the phosphocarrier protein (HPr). Biochimie. 1976;58(4):435–441. doi: 10.1016/s0300-9084(76)80254-4. [DOI] [PubMed] [Google Scholar]
- Marquet M., Creignou M. C., Dedonder R. The phosphoenolpyruvate : methyl-alpha-d-glucoside phosphotransferase system in Bacillus subtilis Marburg : kinetic studies of enzyme ii and evidence for a phosphoryl enzyme ii intermediate. Biochimie. 1978;60(11-12):1283–1287. doi: 10.1016/s0300-9084(79)80445-9. [DOI] [PubMed] [Google Scholar]
- Niaudet B., Gay P., Dedonder R. Identification of the structural gene of the PEP-phosphotransferase enzyme I in Bacillus subtilis Marburg. Mol Gen Genet. 1975;136(4):337–349. doi: 10.1007/BF00341718. [DOI] [PubMed] [Google Scholar]
- Niaudet B., Goze A., Ehrlich S. D. Insertional mutagenesis in Bacillus subtilis: mechanism and use in gene cloning. Gene. 1982 Oct;19(3):277–284. doi: 10.1016/0378-1119(82)90017-8. [DOI] [PubMed] [Google Scholar]
- Perkins J. B., Youngman P. J. Construction and properties of Tn917-lac, a transposon derivative that mediates transcriptional gene fusions in Bacillus subtilis. Proc Natl Acad Sci U S A. 1986 Jan;83(1):140–144. doi: 10.1073/pnas.83.1.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perret J., Gay P. Kinetic study of a phosphoryl exchange reaction between fructose and fructose 1-phosphate catalyzed by the membrane-bound enzyme II of the phosphoenolpyruvate-fructose 1-phosphotransferase system of Bacillus subtilis. Eur J Biochem. 1979 Dec;102(1):237–246. doi: 10.1111/j.1432-1033.1979.tb06285.x. [DOI] [PubMed] [Google Scholar]
- Postma P. W., Lengeler J. W. Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria. Microbiol Rev. 1985 Sep;49(3):232–269. doi: 10.1128/mr.49.3.232-269.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ratet P., Richaud F. Construction and uses of a new transposable element whose insertion is able to produce gene fusions with the neomycin-phosphotransferase-coding region of Tn903. Gene. 1986;42(2):185–192. doi: 10.1016/0378-1119(86)90295-7. [DOI] [PubMed] [Google Scholar]
- Reizer J., Novotny M. J., Hengstenberg W., Saier M. H., Jr Properties of ATP-dependent protein kinase from Streptococcus pyogenes that phosphorylates a seryl residue in HPr, a phosphocarrier protein of the phosphotransferase system. J Bacteriol. 1984 Oct;160(1):333–340. doi: 10.1128/jb.160.1.333-340.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reizer J., Novotny M. J., Stuiver I., Saier M. H., Jr Regulation of glycerol uptake by the phosphoenolpyruvate-sugar phosphotransferase system in Bacillus subtilis. J Bacteriol. 1984 Jul;159(1):243–250. doi: 10.1128/jb.159.1.243-250.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]