Abstract
Conditions for efficient transformation of Amycolatopsis orientalis (Nocardia orientalis) protoplasts by Streptomyces plasmid cloning vectors were identified. Three streptomycete plasmid origins of replication function in A. orientalis, as do the apramycin resistance gene from Escherichia coli, the thiostrepton resistance gene from Streptomyces azureus, and the tyrosinase gene from Streptomyces antibioticus. A. orientalis appears to express some restriction and modification, because highest transformation frequencies (10(6)/micrograms of DNA) were obtained when plasmid pIJ702 was modified by passage in A. orientalis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baltz R. H. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. J Gen Microbiol. 1978 Jul;107(1):93–102. doi: 10.1099/00221287-107-1-93. [DOI] [PubMed] [Google Scholar]
- Baltz R. H., Matsushima P. Advances in protoplast fusion and transformation in Streptomyces. Experientia Suppl. 1983;46:143–148. doi: 10.1007/978-3-0348-6776-4_18. [DOI] [PubMed] [Google Scholar]
- Baltz R. H., Matsushima P. Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration. J Gen Microbiol. 1981 Nov;127(1):137–146. doi: 10.1099/00221287-127-1-137. [DOI] [PubMed] [Google Scholar]
- Barna J. C., Williams D. H. The structure and mode of action of glycopeptide antibiotics of the vancomycin group. Annu Rev Microbiol. 1984;38:339–357. doi: 10.1146/annurev.mi.38.100184.002011. [DOI] [PubMed] [Google Scholar]
- Bibb M. J., Ward J. M., Hopwood D. A. Transformation of plasmid DNA into Streptomyces at high frequency. Nature. 1978 Jul 27;274(5669):398–400. doi: 10.1038/274398a0. [DOI] [PubMed] [Google Scholar]
- Lampel J. S., Strohl W. R. Transformation and transfection of anthracycline-producing streptomycetes. Appl Environ Microbiol. 1986 Jan;51(1):126–131. doi: 10.1128/aem.51.1.126-131.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Larson J. L., Hershberger C. L. The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid. 1986 May;15(3):199–209. doi: 10.1016/0147-619x(86)90038-7. [DOI] [PubMed] [Google Scholar]
- Matsushima P., Baltz R. H. Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts. J Bacteriol. 1985 Jul;163(1):180–185. doi: 10.1128/jb.163.1.180-185.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pidcock K. A., Montenecourt B. S., Sands J. A. Genetic Recombination and Transformation in Protoplasts of Thermomonospora fusca. Appl Environ Microbiol. 1985 Sep;50(3):693–695. doi: 10.1128/aem.50.3.693-695.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rao R. N., Allen N. E., Hobbs J. N., Jr, Alborn W. E., Jr, Kirst H. A., Paschal J. W. Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli. Antimicrob Agents Chemother. 1983 Nov;24(5):689–695. doi: 10.1128/aac.24.5.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richardson M. A., Mabe J. A., Beerman N. E., Nakatsukasa W. M., Fayerman J. T. Development of cloning vehicles from the Streptomyces plasmid pFJ103. Gene. 1982 Dec;20(3):451–457. doi: 10.1016/0378-1119(82)90214-1. [DOI] [PubMed] [Google Scholar]
- Thompson C. J., Ward J. M., Hopwood D. A. Cloning of antibiotic resistance and nutritional genes in streptomycetes. J Bacteriol. 1982 Aug;151(2):668–677. doi: 10.1128/jb.151.2.668-677.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto H., Maurer K. H., Hutchinson C. R. Transformation of Streptomyces erythraeus. J Antibiot (Tokyo) 1986 Sep;39(9):1304–1313. doi: 10.7164/antibiotics.39.1304. [DOI] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]