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. 1987 May;169(5):2307–2309. doi: 10.1128/jb.169.5.2307-2309.1987

Immunoelectron microscopic demonstration of ATPase on the cytoplasmic membrane of the methanogenic bacterium strain Göl.

F Mayer, A Jussofie, M Salzmann, M Lübben, M Rohde, G Gottschalk
PMCID: PMC212165  PMID: 2952638

Abstract

ATPase was shown to be present on the cytoplasmic membrane of the methanogenic bacterium strain Göl. The enzyme was identified by an immunoelectron microscopic technique by using polyclonal antiserum directed against the beta subunit of Escherichia coli F0F1-ATPase. Negatively stained membrane vesicles exhibited a dense population of stalked particles similar in dimensions and fine structure to typical F0F1-ATPase particles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaut M., Gottschalk G. Coupling of ATP synthesis and methane formation from methanol and molecular hydrogen in Methanosarcina barkeri. Eur J Biochem. 1984 May 15;141(1):217–222. doi: 10.1111/j.1432-1033.1984.tb08178.x. [DOI] [PubMed] [Google Scholar]
  2. Daniels L., Sparling R., Sprott G. D. The bioenergetics of methanogenesis. Biochim Biophys Acta. 1984 Sep 6;768(2):113–163. doi: 10.1016/0304-4173(84)90002-8. [DOI] [PubMed] [Google Scholar]
  3. De Mey J., Moeremans M., Geuens G., Nuydens R., De Brabander M. High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method. Cell Biol Int Rep. 1981 Sep;5(9):889–899. doi: 10.1016/0309-1651(81)90204-6. [DOI] [PubMed] [Google Scholar]
  4. Doddema H. J., Hutten T. J., van der Drift C., Vogels G. D. ATP hydrolysis and synthesis by the membrane-bound ATP synthetase complex of Methanobacterium thermoautotrophicum. J Bacteriol. 1978 Oct;136(1):19–23. doi: 10.1128/jb.136.1.19-23.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Futai M., Kanazawa H. Structure and function of proton-translocating adenosine triphosphatase (F0F1): biochemical and molecular biological approaches. Microbiol Rev. 1983 Sep;47(3):285–312. doi: 10.1128/mr.47.3.285-312.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kenealy W. R., Zeikus J. G. One-carbon metabolism in methanogens: evidence for synthesis of a two-carbon cellular intermediate and unification of catabolism and anabolism in Methanosarcina barkeri. J Bacteriol. 1982 Aug;151(2):932–941. doi: 10.1128/jb.151.2.932-941.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kobayashi H., Van Brunt J., Harold F. M. ATP-linked calcium transport in cells and membrane vesicles of Streptococcus faecalis. J Biol Chem. 1978 Apr 10;253(7):2085–2092. [PubMed] [Google Scholar]
  8. Lanyi J. K., MacDonald R. E. Existence of electrogenic hydrogen ion/sodium ion antiport in Halobacterium halobium cell envelope vesicles. Biochemistry. 1976 Oct 19;15(21):4608–4614. doi: 10.1021/bi00666a010. [DOI] [PubMed] [Google Scholar]
  9. Mayer F., Ivey D. M., Ljungdahl L. G. Macromolecular organization of F1-ATPase isolated from Clostridium thermoaceticum as revealed by electron microscopy. J Bacteriol. 1986 Jun;166(3):1128–1130. doi: 10.1128/jb.166.3.1128-1130.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shapiro S., Wolfe R. S. Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri. J Bacteriol. 1980 Feb;141(2):728–734. doi: 10.1128/jb.141.2.728-734.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Slot J. W., Geuze H. J. Sizing of protein A-colloidal gold probes for immunoelectron microscopy. J Cell Biol. 1981 Aug;90(2):533–536. doi: 10.1083/jcb.90.2.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sprott G. D., Jarrell K. F. Sensitivity of methanogenic bacteria to dicyclohexylcarbodiimide. Can J Microbiol. 1982 Aug;28(8):982–986. doi: 10.1139/m82-146. [DOI] [PubMed] [Google Scholar]
  13. Taylor C. D., Wolfe R. S. Structure and methylation of coenzyme M(HSCH2CH2SO3). J Biol Chem. 1974 Aug 10;249(15):4879–4885. [PubMed] [Google Scholar]
  14. Tiedge H., Schäfer G., Mayer F. An electron microscopic approach to the quaternary structure of mitochondrial F1-ATPase. Eur J Biochem. 1983 Apr 15;132(1):37–45. doi: 10.1111/j.1432-1033.1983.tb07322.x. [DOI] [PubMed] [Google Scholar]
  15. Zehnder A. J., Wuhrmann K. Titanium (III) citrate as a nontoxic oxidation-reduction buffering system for the culture of obligate anaerobes. Science. 1976 Dec 10;194(4270):1165–1166. doi: 10.1126/science.793008. [DOI] [PubMed] [Google Scholar]

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