Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Jun;169(6):2730–2738. doi: 10.1128/jb.169.6.2730-2738.1987

Genetic transformation in the methanogen Methanococcus voltae PS.

G Bertani, L Baresi
PMCID: PMC212178  PMID: 3034867

Abstract

Mutations causing requirements for histidine, purine, and vitamin B12 were obtained in strain PS of Methanococcus voltae (archaebacteria) upon irradiation with UV or gamma rays. The first two mutations were shown to revert at low frequencies and were used to demonstrate the occurrence of transformation with homologous, wild-type DNA. The transformation rates obtained for these presumably chromosomal markers were in the range of 2 to 100 transformants per microgram of DNA. Mutants resistant to 2-bromoethanesulfonate and to 5-methyl-DL-tryptophan were also isolated.

Full text

PDF
2730

Images in this article

2733Image
on p.2733

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERTANI G. Sensitivities of different bacteriophage species to ionizing radiations. J Bacteriol. 1960 Mar;79:387–393. doi: 10.1128/jb.79.3.387-393.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cosloy S. D., Oishi M. Genetic transformation in Escherichia coli K12. Proc Natl Acad Sci U S A. 1973 Jan;70(1):84–87. doi: 10.1073/pnas.70.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daniels L., Sparling R., Sprott G. D. The bioenergetics of methanogenesis. Biochim Biophys Acta. 1984 Sep 6;768(2):113–163. doi: 10.1016/0304-4173(84)90002-8. [DOI] [PubMed] [Google Scholar]
  5. Dennis P. P. Molecular biology of archaebacteria. J Bacteriol. 1986 Nov;168(2):471–478. doi: 10.1128/jb.168.2.471-478.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hook E. W., 3rd, Baker-Zander S. A., Moskovitz B. L., Lukehart S. A., Handsfield H. H. Ceftriaxone therapy for asymptomatic neurosyphilis. Case report and Western blot analysis of serum and cerebrospinal fluid IgG response to therapy. Sex Transm Dis. 1986 Jul-Sep;13(3 Suppl):185–188. [PubMed] [Google Scholar]
  7. Jones J. B., Bowers B., Stadtman T. C. Methanococcus vannielii: ultrastructure and sensitivity to detergents and antibiotics. J Bacteriol. 1977 Jun;130(3):1357–1363. doi: 10.1128/jb.130.3.1357-1363.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones W. J., Whitman W. B., Fields R. D., Wolfe R. S. Growth and plating efficiency of methanococci on agar media. Appl Environ Microbiol. 1983 Jul;46(1):220–226. doi: 10.1128/aem.46.1.220-226.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klein A., Schnorr M. Genome complexity of methanogenic bacteria. J Bacteriol. 1984 May;158(2):628–631. doi: 10.1128/jb.158.2.628-631.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  11. McBride B. C., Wolfe R. S. A new coenzyme of methyl transfer, coenzyme M. Biochemistry. 1971 Jun 8;10(12):2317–2324. doi: 10.1021/bi00788a022. [DOI] [PubMed] [Google Scholar]
  12. Meile L., Kiener A., Leisinger T. A plasmid in the archaebacterium Methanobacterium thermoautotrophicum. Mol Gen Genet. 1983;191(3):480–484. doi: 10.1007/BF00425766. [DOI] [PubMed] [Google Scholar]
  13. Reeve A., Shulman S. A., Zimmerman A. W., Cassidy S. B. Methylphenidate therapy for aggression in a man with ring 22 chromosome. Report and literature review. Arch Neurol. 1985 Jan;42(1):69–72. doi: 10.1001/archneur.1985.04060010075019. [DOI] [PubMed] [Google Scholar]
  14. Santoro N., Konisky J. Characterization of bromoethanesulfonate-resistant mutants of Methanococcus voltae: evidence of a coenzyme M transport system. J Bacteriol. 1987 Feb;169(2):660–665. doi: 10.1128/jb.169.2.660-665.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith M. R., Lequerica J. L. Methanosarcina mutant unable to produce methane or assimilate carbon from acetate. J Bacteriol. 1985 Nov;164(2):618–625. doi: 10.1128/jb.164.2.618-625.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Whitman W. B., Ankwanda E., Wolfe R. S. Nutrition and carbon metabolism of Methanococcus voltae. J Bacteriol. 1982 Mar;149(3):852–863. doi: 10.1128/jb.149.3.852-863.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Woese C. R., Fox G. E. Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088–5090. doi: 10.1073/pnas.74.11.5088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wood A. G., Redborg A. H., Cue D. R., Whitman W. B., Konisky J. Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae. J Bacteriol. 1983 Oct;156(1):19–29. doi: 10.1128/jb.156.1.19-29.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wood A. G., Whitman W. B., Konisky J. A newly-isolated marine methanogen harbors a small cryptic plasmid. Arch Microbiol. 1985 Aug;142(3):259–261. doi: 10.1007/BF00693400. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES