Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Jun;169(6):2885–2888. doi: 10.1128/jb.169.6.2885-2888.1987

An inducible pathway is required for mutagenesis in Salmonella typhimurium LT2.

C Orrego, E Eisenstadt
PMCID: PMC212206  PMID: 3294811

Abstract

UV mutability of Salmonella typhimurium LT2 was eliminated in the presence of a multicopy plasmid carrying the Escherichia coli lexA+ gene. This result suggests that inducible, SOS-like functions are required for UV mutagenesis in S. typhimurium. S. typhimurium strains carrying either point or deletion mutations in topA had previously been shown to lose their mutability by UV or methyl methanesulfonate (K. Overbye and P. Margolin, J. Bacteriol. 146:170-178, 1981; K. Overbye, S. M. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Mitomycin C induction of the phi(mucB'-lacZ') fusion (a DNA damage-inducible locus carried on plasmid pSE205) in S. typhimurium topA was normal, suggesting that RecA is activated in topA mutants. These observations lead us to deduce that S. typhimurium has at least one DNA damage-inducible locus in addition to recA that is required for UV mutability.

Full text

PDF
2885

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagg A., Kenyon C. J., Walker G. C. Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5749–5753. doi: 10.1073/pnas.78.9.5749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blanco M., Herrera G., Collado P., Rebollo J. E., Botella L. M. Influence of RecA protein on induced mutagenesis. Biochimie. 1982 Aug-Sep;64(8-9):633–636. doi: 10.1016/s0300-9084(82)80102-8. [DOI] [PubMed] [Google Scholar]
  3. Brent R., Ptashne M. The lexA gene product represses its own promoter. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1932–1936. doi: 10.1073/pnas.77.4.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bullas L. R., Ryu J. I. Salmonella typhimurium LT2 strains which are r- m+ for all three chromosomally located systems of DNA restriction and modification. J Bacteriol. 1983 Oct;156(1):471–474. doi: 10.1128/jb.156.1.471-474.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dobson P. P., Walker G. C. Plasmid (pKM101)-mediated Weigle reactivation in Escherichia coli K12 and Salmonella typhimurium LT2: genetic dependence, kinetics of induction, and effect of chloramphenicol. Mutat Res. 1980 Jun;71(1):25–41. doi: 10.1016/0027-5107(80)90004-4. [DOI] [PubMed] [Google Scholar]
  6. Drlica K. Biology of bacterial deoxyribonucleic acid topoisomerases. Microbiol Rev. 1984 Dec;48(4):273–289. doi: 10.1128/mr.48.4.273-289.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elledge S. J., Walker G. C. The muc genes of pKM101 are induced by DNA damage. J Bacteriol. 1983 Sep;155(3):1306–1315. doi: 10.1128/jb.155.3.1306-1315.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ennis D. G., Fisher B., Edmiston S., Mount D. W. Dual role for Escherichia coli RecA protein in SOS mutagenesis. Proc Natl Acad Sci U S A. 1985 May;82(10):3325–3329. doi: 10.1073/pnas.82.10.3325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kanazir D. T., Hartman P. E., Savic D. Ultraviolet-induced reversions of Salmonella his frameshift mutations. J Bacteriol. 1970 Feb;101(2):649–651. doi: 10.1128/jb.101.2.649-651.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kato T., Shinoura Y. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Mol Gen Genet. 1977 Nov 14;156(2):121–131. doi: 10.1007/BF00283484. [DOI] [PubMed] [Google Scholar]
  11. Lee G. S., Ames G. F. Analysis of promoter mutations in the histidine transport operon of Salmonella typhimurium: use of hybrid M13 bacteriophages for cloning, transformation, and sequencing. J Bacteriol. 1984 Sep;159(3):1000–1005. doi: 10.1128/jb.159.3.1000-1005.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MacPhee D. G. Effect of rec mutations on the ultraviolet protecting and mutation-enhancing properties of the plasmid R-Utrecht in Salmonella typhimurium. Mutat Res. 1973 Sep;19(3):357–359. doi: 10.1016/0027-5107(73)90237-6. [DOI] [PubMed] [Google Scholar]
  13. MacPhee D. G. Effects of an R factor and caffeine on ultraviolet mutability in Salmonella typhimurium. Mutat Res. 1973 Jun;18(3):367–370. doi: 10.1016/0027-5107(73)90221-2. [DOI] [PubMed] [Google Scholar]
  14. Margolin P., Zumstein L., Sternglanz R., Wang J. C. The Escherichia coli supX locus is topA, the structural gene for DNA topoisomerase I. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5437–5441. doi: 10.1073/pnas.82.16.5437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McCann J., Spingarn N. E., Kobori J., Ames B. N. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci U S A. 1975 Mar;72(3):979–983. doi: 10.1073/pnas.72.3.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miller S. S., Eisenstadt E. Enhanced sensitivity of Escherichia coli umuC to photodynamic inactivation by angelicin (isopsoralen). J Bacteriol. 1985 Jun;162(3):1307–1310. doi: 10.1128/jb.162.3.1307-1310.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mortelmans K. E., Stocker B. A. Ultraviolet light protection, enhancement of ultraviolet light mutagenesis, and mutator effect of plasmid R46 in Salmonella typhimurium. J Bacteriol. 1976 Oct;128(1):271–282. doi: 10.1128/jb.128.1.271-282.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nakano E., Ichikawa-Ryo H., Kondo S. Comparative mutability of the Ames tester strains of Salmonella typhimurium by ultraviolet radiation and by 4-nitroquinoline 1-oxide. Mutat Res. 1982 Mar;93(1):35–44. doi: 10.1016/0027-5107(82)90123-3. [DOI] [PubMed] [Google Scholar]
  19. Overbye K. M., Basu S. K., Margolin P. Loss of DNA topoisomerase I activity alters many cellular functions in Salmonella typhimurium. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):785–791. doi: 10.1101/sqb.1983.047.01.090. [DOI] [PubMed] [Google Scholar]
  20. Overbye K. M., Margolin P. Role of the supX gene in ultraviolet light-induced mutagenesis in Salmonella typhimurium. J Bacteriol. 1981 Apr;146(1):170–178. doi: 10.1128/jb.146.1.170-178.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pang P. P., Walker G. C. The Salmonella typhimurium LT2 uvrD gene is regulated by the lexA gene product. J Bacteriol. 1983 Jun;154(3):1502–1504. doi: 10.1128/jb.154.3.1502-1504.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Perry K. L., Elledge S. J., Mitchell B. B., Marsh L., Walker G. C. umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4331–4335. doi: 10.1073/pnas.82.13.4331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pierré A., Paoletti C. Purification and characterization of recA protein from salmonella typhimurium. J Biol Chem. 1983 Mar 10;258(5):2870–2874. [PubMed] [Google Scholar]
  24. Ruiz-Rubio M., Woodgate R., Bridges B. A., Herrera G., Blanco M. New role for photoreversible pyrimidine dimers in induction of prototrophic mutations in excision-deficient Escherichia coli by UV light. J Bacteriol. 1986 Jun;166(3):1141–1143. doi: 10.1128/jb.166.3.1141-1143.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Savić D. J., Kanazir D. T. The effect of a histidine operator-constitutive mutation on UV-induced mutability within the histidine operon of Salmonella typhimurium. Mol Gen Genet. 1972;118(1):45–50. doi: 10.1007/BF02428331. [DOI] [PubMed] [Google Scholar]
  26. Sedgwick S. G., Goodwin P. A. Differences in mutagenic and recombinational DNA repair in enterobacteria. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4172–4176. doi: 10.1073/pnas.82.12.4172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sedgwick S. G., Goodwin P. A. Interspecies regulation of the SOS response by the E. coli lexA+ gene. Mutat Res. 1985 May;145(3):103–106. doi: 10.1016/0167-8817(85)90015-x. [DOI] [PubMed] [Google Scholar]
  28. Skavronskaya A. G., Stepanova N. F., Andreeva I. V. UV-mutable hybrids of Salmonella incorporating Escherichia coli region adjacent to tryptophan operon. Mol Gen Genet. 1982;185(2):315–318. doi: 10.1007/BF00330804. [DOI] [PubMed] [Google Scholar]
  29. Walker G. C. Inducible DNA repair systems. Annu Rev Biochem. 1985;54:425–457. doi: 10.1146/annurev.bi.54.070185.002233. [DOI] [PubMed] [Google Scholar]
  30. Walker G. C. Inducible reactivation and mutagenesis of UV-irradiated bacteriophage P22 in Salmonella typhimurium LT2 containing the plasmid pKM101. J Bacteriol. 1978 Aug;135(2):415–421. doi: 10.1128/jb.135.2.415-421.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Walker G. C. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984 Mar;48(1):60–93. doi: 10.1128/mr.48.1.60-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. ZINDER N. D., LEDERBERG J. Genetic exchange in Salmonella. J Bacteriol. 1952 Nov;64(5):679–699. doi: 10.1128/jb.64.5.679-699.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES