Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Jun;169(6):2899–2901. doi: 10.1128/jb.169.6.2899-2901.1987

Evidence that colicin X is microcin B17.

J L San Millán, R Kolter, F Moreno
PMCID: PMC212210  PMID: 3034869

Abstract

The DNA replication inhibitor microcin B17 is a peptide antibiotic produced by Escherichia coli strains carrying plasmid pMccB17. Here we present evidence that antibiotic activities previously named colicin X are probably identical to microcin B17. Our results include comparison of the conditions of production of the antibiotics, their mode of action, cross-immunity of producer strains, and cross-resistance of resistant mutants. Plasmids encoding colicin X have been identified and shown to have a region of significant homology with the microcin B17-producing region of pMccB17 DNA.

Full text

PDF
2899

Images in this article

2900Image
on p.2900

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguilar A., Pérez-Díaz J. C., Baquero F., Asensio C. Microcin 15m from Escherichia coli: mechanism of antibiotic action. Antimicrob Agents Chemother. 1982 Mar;21(3):381–386. doi: 10.1128/aac.21.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baquero F., Bouanchaud D., Martinez-Perez M. C., Fernandez C. Microcin plasmids: a group of extrachromosomal elements coding for low-molecular-weight antibiotics in Escherichia coli. J Bacteriol. 1978 Aug;135(2):342–347. doi: 10.1128/jb.135.2.342-347.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davagnino J., Herrero M., Furlong D., Moreno F., Kolter R. The DNA replication inhibitor microcin B17 is a forty-three-amino-acid protein containing sixty percent glycine. Proteins. 1986 Nov;1(3):230–238. doi: 10.1002/prot.340010305. [DOI] [PubMed] [Google Scholar]
  4. Davies J. K., Reeves P. Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A. J Bacteriol. 1975 Jul;123(1):102–117. doi: 10.1128/jb.123.1.102-117.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guarente L. P., Isberg R. R., Syvanen M., Silhavy T. J. Conferral of transposable properties to a chromosomal gene in Escherichia coli. J Mol Biol. 1980 Aug 15;141(3):235–248. doi: 10.1016/0022-2836(80)90179-5. [DOI] [PubMed] [Google Scholar]
  6. Herrero M., Moreno F. Microcin B17 blocks DNA replication and induces the SOS system in Escherichia coli. J Gen Microbiol. 1986 Feb;132(2):393–402. doi: 10.1099/00221287-132-2-393. [DOI] [PubMed] [Google Scholar]
  7. Lanka E., Lurz R., Fürste J. P. Molecular cloning and mapping of SphI restriction fragments of plasmid RP4. Plasmid. 1983 Nov;10(3):303–307. doi: 10.1016/0147-619x(83)90047-1. [DOI] [PubMed] [Google Scholar]
  8. Laviña M., Pugsley A. P., Moreno F. Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12. J Gen Microbiol. 1986 Jun;132(6):1685–1693. doi: 10.1099/00221287-132-6-1685. [DOI] [PubMed] [Google Scholar]
  9. Novoa M. A., Díaz-Guerra L., San Millán J. L., Moreno F. Cloning and mapping of the genetic determinants for microcin C7 production and immunity. J Bacteriol. 1986 Dec;168(3):1384–1391. doi: 10.1128/jb.168.3.1384-1391.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PAPAVASSILIOU J. Biological characteristics of colicine X. Nature. 1961 Apr 1;190:110–110. doi: 10.1038/190110a0. [DOI] [PubMed] [Google Scholar]
  11. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  12. San Millan J. L., Hernandez-Chico C., Pereda P., Moreno F. Cloning and mapping of the genetic determinants for microcin B17 production and immunity. J Bacteriol. 1985 Jul;163(1):275–281. doi: 10.1128/jb.163.1.275-281.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Yang C. C., Konisky J. Colicin V-treated Escherichia coli does not generate membrane potential. J Bacteriol. 1984 May;158(2):757–759. doi: 10.1128/jb.158.2.757-759.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES