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. 1987 Jul;169(7):2932–2937. doi: 10.1128/jb.169.7.2932-2937.1987

Glucose transport in a kinaseless Saccharomyces cerevisiae mutant.

J M Lang, V P Cirillo
PMCID: PMC212329  PMID: 3298207

Abstract

Wild-type Saccharomyces cerevisiae organisms contain three kinases which catalyze the phosphorylation of glucose: two hexokinase isozymes (PI and PII) and one glucokinase. Glucose transport measurements for triple-kinaseless mutants, which lack all three of these kinases, confirm that the kinases are involved in the low apparent Km transport process observed in metabolizing cells. Thus kinase-positive cells containing one or more of the three kinases exhibit biphasic transport kinetics with a low apparent Km (1 to 2 mM) and high apparent Km (40 to 50 mM) component. Triple-kinaseless cells, however, exhibit only the high apparent Km component of kinase-positive cells (60 mM). Kinetic analysis of glucose transport in the triple-kinaseless cells shows that glucose is transported by a facilitated diffusion process which exhibits trans-stimulated equilibrium exchange and influx counterflow.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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