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. 1987 Jul;169(7):3013–3022. doi: 10.1128/jb.169.7.3013-3022.1987

Bacteriophages of Saccharopolyspora erythraea.

A D Grund, C R Hutchinson
PMCID: PMC212342  PMID: 3036768

Abstract

Five bacteriophages infecting only Saccharopolyspora erythraea (formerly Streptomyces erythreus) among 43 Streptomyces spp. tested were classified into two groups by phage-host relationships, restriction enzyme mapping, cohesive-end determinations, and Southern hybridizations. phi SE6, the most frequently isolated phage, produced clear plaques on all hosts tested, while phi SE45, phi SE57, phi SE60, and phi SE69 produced turbid plaques. phi SE6 DNA was linear, had a molecular weight of (27.6 +/- 1) X 10(6) and, like the DNAs of phi SE45, phi SE57, and phi SE69, lacked cohesive ends. The characteristic patterns of of ClaI and HindIII restriction digests of phi SE6 DNA and the results of Southern hybridizations with three different ClaI fragments of phi SE6 DNA as probes indicated that phi SE6 DNA was partially circularly permuted and terminally redundant, suggesting that it was packaged by a headful packaging mechanism. Southern hybridization data also showed that phi SE45, phi SE57, and phi SE69 were closely related to phi SE6. phi SE60 DNA, in contrast, had cohesive ends, and restriction mapping plus Southern hybridization data showed that phi SE60 was unrelated to the other four phages.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brzezinski R., Surmacz E., Kutner M., Piekarowicz A. Restriction mapping and close relationship of the DNA of Streptomyces erythraeus phages 121 and SE-5. J Gen Microbiol. 1986 Oct;132(10):2937–2943. doi: 10.1099/00221287-132-10-2937. [DOI] [PubMed] [Google Scholar]
  4. Chater K. F., Hopwood D. A., Kieser T., Thompson C. J. Gene cloning in Streptomyces. Curr Top Microbiol Immunol. 1982;96:69–95. doi: 10.1007/978-3-642-68315-2_5. [DOI] [PubMed] [Google Scholar]
  5. Cox K. L., Baltz R. H. Restriction of bacteriophage plaque formation in Streptomyces spp. J Bacteriol. 1984 Aug;159(2):499–504. doi: 10.1128/jb.159.2.499-504.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis R. W., Parkinson J. S. Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi. J Mol Biol. 1971 Mar 14;56(2):403–423. doi: 10.1016/0022-2836(71)90473-6. [DOI] [PubMed] [Google Scholar]
  7. Donadio S., Paladino R., Costanzi I., Sparapani P., Schreil W., Iaccarino M. Characterization of bacteriophages infecting Streptomyces erythreus and properties of phage-resistant mutants. J Bacteriol. 1986 Jun;166(3):1055–1060. doi: 10.1128/jb.166.3.1055-1060.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Inman R. B., Schnös M. Structure of branch points in replicating DNA: presence of single-stranded connections in lambda DNA branch points. J Mol Biol. 1971 Mar 14;56(2):319–325. doi: 10.1016/0022-2836(71)90467-0. [DOI] [PubMed] [Google Scholar]
  9. Jackson E. N., Jackson D. A., Deans R. J. EcoRI analysis of bacteriophage P22 DNA packaging. J Mol Biol. 1978 Jan 25;118(3):365–388. doi: 10.1016/0022-2836(78)90234-6. [DOI] [PubMed] [Google Scholar]
  10. Lomovskaya N. D., Chater K. F., Mkrtumian N. M. Genetics and molecular biology of Streptomyces bacteriophages. Microbiol Rev. 1980 Jun;44(2):206–229. doi: 10.1128/mr.44.2.206-229.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Parkinson J. S., Huskey R. J. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J Mol Biol. 1971 Mar 14;56(2):369–384. doi: 10.1016/0022-2836(71)90471-2. [DOI] [PubMed] [Google Scholar]
  12. RAUTENSHTEIN Ia I., TIKHONENKO A. S., RETINSKAIA V. I. [Electron-microscopic studies on actinophages of lysogenic Act. erythreus cultures]. Mikrobiologiia. 1962 Jan-Feb;31:49–53. [PubMed] [Google Scholar]
  13. Rodicio M. R., Chater K. F. Small DNA-free liposomes stimulate transfection of streptomyces protoplasts. J Bacteriol. 1982 Sep;151(3):1078–1085. doi: 10.1128/jb.151.3.1078-1085.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schnös M., Inman R. B. Caffeine-induced re-initiation of phage lambda DNA replication. J Mol Biol. 1982 Aug 15;159(3):457–465. doi: 10.1016/0022-2836(82)90294-7. [DOI] [PubMed] [Google Scholar]
  15. Susskind M. M., Botstein D. Molecular genetics of bacteriophage P22. Microbiol Rev. 1978 Jun;42(2):385–413. doi: 10.1128/mr.42.2.385-413.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tye B. K., Chan R. K., Botstein D. Packaging of an oversize transducing genome by Salmonella phage P22. J Mol Biol. 1974 Jan 5;85(4):485–500. doi: 10.1016/0022-2836(74)90311-8. [DOI] [PubMed] [Google Scholar]
  17. Tye B. K., Huberman J. A., Botstein D. Non-random circular permutation of phage P22 DNA. J Mol Biol. 1974 Jan 5;85(4):501–528. doi: 10.1016/0022-2836(74)90312-x. [DOI] [PubMed] [Google Scholar]
  18. Weber J. M., Wierman C. K., Hutchinson C. R. Genetic analysis of erythromycin production in Streptomyces erythreus. J Bacteriol. 1985 Oct;164(1):425–433. doi: 10.1128/jb.164.1.425-433.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yamamoto H., Maurer K. H., Hutchinson C. R. Transformation of Streptomyces erythraeus. J Antibiot (Tokyo) 1986 Sep;39(9):1304–1313. doi: 10.7164/antibiotics.39.1304. [DOI] [PubMed] [Google Scholar]

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