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. 1987 Jul;169(7):3082–3087. doi: 10.1128/jb.169.7.3082-3087.1987

Translational fusion with a secretory enzyme as an indicator.

P Z Wang, S J Projan, K R Leason, R P Novick
PMCID: PMC212352  PMID: 3496329

Abstract

A novel type of translational fusion system has been developed by using a secretory protein, staphylococcal beta-lactamase, as an indicator. The beta-lactamase structural gene was modified to provide N-terminal extensions of 13 and 162 amino acids, and in both cases, the fusion protein was processed and the mature active enzyme was secreted; thus, the expression of a particular upstream gene can be analyzed by monitoring the beta-lactamase activity.

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Selected References

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