Abstract
The nucleotide sequences of the single genes coding for the B-type small, acid-soluble spore proteins (SASP) of Bacillus cereus, B. stearothermophilus, and "Thermoactinomyces thalpophilus" were determined, and the amino acid sequences of all B-type SASP were compared. While this type of SASP showed significant sequence conservation around the two spore protease cleavage sites, alignment of these sequences required the introduction of gaps, and even then only 19 of the residues were conserved exactly in all five proteins. However, all five B-type SASP did contain a large (27 to 35-residue), rather well-conserved amino acid sequence repeat, and four of the five proteins had well-conserved regions of 14 to 17 amino acids which appeared three times.
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- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Connors M. J., Howard S., Hoch J., Setlow P. Determination of the chromosomal locations of four Bacillus subtilis genes which code for a family of small, acid-soluble spore proteins. J Bacteriol. 1986 May;166(2):412–416. doi: 10.1128/jb.166.2.412-416.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fliss E. R., Loshon C. A., Setlow P. Genes for Bacillus megaterium small, acid-soluble spore proteins: cloning and nucleotide sequence of three additional genes from this multigene family. J Bacteriol. 1986 Feb;165(2):467–473. doi: 10.1128/jb.165.2.467-473.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hackett R. H., Setlow B., Setlow P. Cloning and nucleotide sequence of the Bacillus megaterium gene coding for small, acid-soluble spore protein B. J Bacteriol. 1986 Nov;168(2):1023–1025. doi: 10.1128/jb.168.2.1023-1025.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hackett R. H., Setlow P. Cloning, nucleotide sequencing, and genetic mapping of the gene for small, acid-soluble spore protein gamma of Bacillus subtilis. J Bacteriol. 1987 May;169(5):1985–1992. doi: 10.1128/jb.169.5.1985-1992.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loshon C. A., Fliss E. R., Setlow B., Foerster H. F., Setlow P. Cloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus". J Bacteriol. 1986 Jul;167(1):168–173. doi: 10.1128/jb.167.1.168-173.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mason J. M., Setlow P. Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light. J Bacteriol. 1986 Jul;167(1):174–178. doi: 10.1128/jb.167.1.174-178.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Yuan K., Johnson W. C., Tipper D. J., Setlow P. Comparison of various properties of low-molecular-weight proteins from dormant spores of several Bacillus species. J Bacteriol. 1981 Jun;146(3):965–971. doi: 10.1128/jb.146.3.965-971.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]