Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Aug;169(8):3515–3520. doi: 10.1128/jb.169.8.3515-3520.1987

Change of membrane potential is not a component of the photophobic transduction chain in Halobacterium halobium.

D Oesterhelt, W Marwan
PMCID: PMC212426  PMID: 3611021

Abstract

Long (20 to 50 microns) and bipolarly flagellated cells of Halobacterium halobium were stimulated locally by a focused beam of light, and the photophobic response was analyzed. The results demonstrate that two flagellar bundles did not react in a coordinated fashion. The light-induced stop response of a flagellar bundle only occurred if the stimulus was applied within 5 microns of the polar region. This excluded membrane potential changes from being causally involved in photophobic signalling and indicated that there is a diffusible messenger in the signal transduction chain which is subjected to decay. In addition, the photoreceptor may be localized at the polar end of the cell.

Full text

PDF
3515

Images in this article

3517Image
on p.3517
3518Image
on p.3518

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alam M., Oesterhelt D. Morphology, function and isolation of halobacterial flagella. J Mol Biol. 1984 Jul 15;176(4):459–475. doi: 10.1016/0022-2836(84)90172-4. [DOI] [PubMed] [Google Scholar]
  2. Bogomolni R. A., Spudich J. L. Identification of a third rhodopsin-like pigment in phototactic Halobacterium halobium. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6250–6254. doi: 10.1073/pnas.79.20.6250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ehrlich B. E., Schen C. R., Spudich J. L. Bacterial rhodopsins monitored with fluorescent dyes in vesicles and in vivo. J Membr Biol. 1984;82(1):89–94. doi: 10.1007/BF01870735. [DOI] [PubMed] [Google Scholar]
  4. Forterre P., Elie C., Kohiyama M. Aphidicolin inhibits growth and DNA synthesis in halophilic arachaebacteria. J Bacteriol. 1984 Aug;159(2):800–802. doi: 10.1128/jb.159.2.800-802.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goulbourne E. A., Jr, Greenberg E. P. Chemotaxis of Spirochaeta aurantia: involvement of membrane potential in chemosensory signal transduction. J Bacteriol. 1981 Dec;148(3):837–844. doi: 10.1128/jb.148.3.837-844.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hazemoto N., Kamo N., Kobatake Y., Tsuda M., Terayama Y. Effect of salt on photocycle and ion-pumping of halorhodopsin and third rhodopsinlike pigment of Halobacterium halobium. Biophys J. 1984 Jun;45(6):1073–1077. doi: 10.1016/S0006-3495(84)84254-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hildebrand E., Dencher N. Two photosystems controlling behavioural responses of Halobacterium halobium. Nature. 1975 Sep 4;257(5521):46–48. doi: 10.1038/257046a0. [DOI] [PubMed] [Google Scholar]
  8. Ishihara A., Segall J. E., Block S. M., Berg H. C. Coordination of flagella on filamentous cells of Escherichia coli. J Bacteriol. 1983 Jul;155(1):228–237. doi: 10.1128/jb.155.1.228-237.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Krieg N. R., Tomelty J. P., Wells J. S., Jr Inhibitio of flagellar coordination in Spirillum volutans. J Bacteriol. 1967 Nov;94(5):1431–1436. doi: 10.1128/jb.94.5.1431-1436.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee A. G., Fitzsimons J. T. Motility in normal and filamentous forms of Rhodospirillum rubrum. J Gen Microbiol. 1976 Apr;93(2):346–354. doi: 10.1099/00221287-93-2-346. [DOI] [PubMed] [Google Scholar]
  11. Macnab R. M., Han D. P. Asynchronous switching of flagellar motors on a single bacterial cell. Cell. 1983 Jan;32(1):109–117. doi: 10.1016/0092-8674(83)90501-9. [DOI] [PubMed] [Google Scholar]
  12. Margolin Y., Eisenbach M. Voltage clamp effects on bacterial chemotaxis. J Bacteriol. 1984 Aug;159(2):605–610. doi: 10.1128/jb.159.2.605-610.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marwan W., Oesterhelt D. Signal formation in the halobacterial photophobic response mediated by a fourth retinal protein (P480). J Mol Biol. 1987 May 20;195(2):333–342. doi: 10.1016/0022-2836(87)90654-1. [DOI] [PubMed] [Google Scholar]
  14. Oesterhelt D., Krippahl G. Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants. Ann Microbiol (Paris) 1983 Jul-Aug;134B(1):137–150. doi: 10.1016/s0769-2609(83)80101-x. [DOI] [PubMed] [Google Scholar]
  15. Segall J. E., Ishihara A., Berg H. C. Chemotactic signaling in filamentous cells of Escherichia coli. J Bacteriol. 1985 Jan;161(1):51–59. doi: 10.1128/jb.161.1.51-59.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spudich J. L., Bogomolni R. A. Mechanism of colour discrimination by a bacterial sensory rhodopsin. Nature. 1984 Dec 6;312(5994):509–513. doi: 10.1038/312509a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wolff E. K., Bogomolni R. A., Scherrer P., Hess B., Stoeckenius W. Color discrimination in halobacteria: spectroscopic characterization of a second sensory receptor covering the blue-green region of the spectrum. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7272–7276. doi: 10.1073/pnas.83.19.7272. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES