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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1915 Mar 1;21(3):213–220. doi: 10.1084/jem.21.3.213

NOTES ON THE CULTIVATION OF TREPONEMA PALLIDUM

Hans Zinsser 1, J G Hopkins 1, Ruth Gilbert 1
PMCID: PMC2125285  PMID: 19867864

Abstract

We consider themost importantcontribution reported in this paper the fact that Treponema pallidum can be cultivated in fluid media, without the addition of agar, together with tissues sterilized by heat. This forms an excellent method of obtaining mass cultures for luetin preparation and immunological experimentation. We may add that while the tissue varieties employed have all stongly favored the growth of the treponemata, we have noticed especially active and motile cultures when lung and suprarenal tissues were employed.

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Selected References

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  1. Noguchi H. METHOD FOR THE PURE CULTIVATION OF PATHOGENIC TREPONEMA PALLIDUM (SPIROCHAETA PALLIDA). J Exp Med. 1911 Aug 1;14(2):99–108. doi: 10.1084/jem.14.2.99. [DOI] [PMC free article] [PubMed] [Google Scholar]

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