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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1915 Apr 1;21(4):389–400. doi: 10.1084/jem.21.4.389

CONCENTRATION OF THE PROTECTIVE BODIES IN ANTIPNEUMOCOCCUS SERUM BY MEANS OF SPECIFIC PRECIPITATION

Frederick P Gay 1, Henry T Chickering 1
PMCID: PMC2125319  PMID: 19867879

Abstract

The addition of a water-clear extract of pneumococci to homologous antiserum produces a voluminous precipitate which carries down with it the agglutinins and practically the totality of the protective bodies against pneumococcus infection in animals. This precipitate when washed and resuspended in saline solution to the original volume of serum protects as well as the whole serum. The protein content of such solutions has varied from 0.09 to 0.34 per cent., as contrasted with about 6 per cent. in the original serum. The solution of this precipitate is not necessary to insure protection, and when produced by dilute alkali (sodium hydrate) frequently destroys the immune bodies.

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