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. 1991 Dec;173(24):7841–7847. doi: 10.1128/jb.173.24.7841-7847.1991

A gene cluster required for coordinated biosynthesis of lipopolysaccharide and extracellular polysaccharide also affects virulence of Pseudomonas solanacearum.

C C Kao 1, L Sequeira 1
PMCID: PMC212575  PMID: 1744040

Abstract

Bacterial cell surface components can be important determinants of virulence. At least three gene clusters important for extracellular polysaccharide (EPS) biosynthesis have been previously identified in the plant pathogen Pseudomonas solanacearum. We have found that one of these gene clusters, named ops, is also required for lipopolysaccharide (LPS) biosynthesis. Mutations in any complementation unit of this cluster decreased EPS production, prevented the binding of an LPS-specific phage, and altered the mobility of purified LPS in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, restoration of LPS biosynthesis alone was not sufficient to restore virulence to the wild-type level, suggesting that EPS is important for pathogenesis.

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Selected References

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  1. Ayers A. R., Ayers S. B., Goodman R. N. Extracellular Polysaccharide of Erwinia amylovora: a Correlation with Virulence. Appl Environ Microbiol. 1979 Oct;38(4):659–666. doi: 10.1128/aem.38.4.659-666.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Collins L. V., Hackett J. Molecular cloning, characterization, and nucleotide sequence of the rfc gene, which encodes an O-antigen polymerase of Salmonella typhimurium. J Bacteriol. 1991 Apr;173(8):2521–2529. doi: 10.1128/jb.173.8.2521-2529.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cook D., Sequeira L. Genetic and biochemical characterization of a Pseudomonas solanacearum gene cluster required for extracellular polysaccharide production and for virulence. J Bacteriol. 1991 Mar;173(5):1654–1662. doi: 10.1128/jb.173.5.1654-1662.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coplin D. L., Cook D. Molecular genetics of extracellular polysaccharide biosynthesis in vascular phytopathogenic bacteria. Mol Plant Microbe Interact. 1990 Sep-Oct;3(5):271–279. doi: 10.1094/mpmi-3-271. [DOI] [PubMed] [Google Scholar]
  5. Crowell D. N., Reznikoff W. S., Raetz C. R. Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase. J Bacteriol. 1987 Dec;169(12):5727–5734. doi: 10.1128/jb.169.12.5727-5734.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Djordjevic S. P., Chen H., Batley M., Redmond J. W., Rolfe B. G. Nitrogen fixation ability of exopolysaccharide synthesis mutants of Rhizobium sp. strain NGR234 and Rhizobium trifolii is restored by the addition of homologous exopolysaccharides. J Bacteriol. 1987 Jan;169(1):53–60. doi: 10.1128/jb.169.1.53-60.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Drigues P., Demery-Lafforgue D., Trigalet A., Dupin P., Samain D., Asselineau J. Comparative studies of lipopolysaccharide and exopolysaccharide from a virulent strain of Pseudomonas solanacearum and from three avirulent mutants. J Bacteriol. 1985 May;162(2):504–509. doi: 10.1128/jb.162.2.504-509.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duvick J. P., Sequeira L. Interaction of Pseudomonas solanacearum Lipopolysaccharide and Extracellular Polysaccharide with Agglutinin from Potato Tubers. Appl Environ Microbiol. 1984 Jul;48(1):192–198. doi: 10.1128/aem.48.1.192-198.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hancock R. E., Reeves P. Lipopolysaccharide-deficient, bacteriophage-resistant mutants of Escherichia coli K-12. J Bacteriol. 1976 Jul;127(1):98–108. doi: 10.1128/jb.127.1.98-108.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hendrick C. A., Sequeira L. Lipopolysaccharide-Defective Mutants of the Wilt Pathogen Pseudomonas solanacearum. Appl Environ Microbiol. 1984 Jul;48(1):94–101. doi: 10.1128/aem.48.1.94-101.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoiseth S. K., Connelly C. J., Moxon E. R. Genetics of spontaneous, high-frequency loss of b capsule expression in Haemophilus influenzae. Infect Immun. 1985 Aug;49(2):389–395. doi: 10.1128/iai.49.2.389-395.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jiang X. M., Neal B., Santiago F., Lee S. J., Romana L. K., Reeves P. R. Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol Microbiol. 1991 Mar;5(3):695–713. doi: 10.1111/j.1365-2958.1991.tb00741.x. [DOI] [PubMed] [Google Scholar]
  13. Kadam S. K., Peppler M. S., Sanderson K. E. Temperature-sensitive mutants in rfaI and rfaJ, genes for galactosyltransferase I and glucosyltransferase II, for synthesis of lipopolysaccharide in Salmonella typhimurium. Can J Microbiol. 1985 Sep;31(9):861–869. doi: 10.1139/m85-160. [DOI] [PubMed] [Google Scholar]
  14. Kadam S. K., Rehemtulla A., Sanderson K. E. Cloning of rfaG, B, I, and J genes for glycosyltransferase enzymes for synthesis of the lipopolysaccharide core of Salmonella typhimurium. J Bacteriol. 1985 Jan;161(1):277–284. doi: 10.1128/jb.161.1.277-284.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kamoun S., Cooley M. B., Rogowsky P. M., Kado C. I. Two chromosomal loci involved in production of exopolysaccharide in Agrobacterium tumefaciens. J Bacteriol. 1989 Mar;171(3):1755–1759. doi: 10.1128/jb.171.3.1755-1759.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kao C., Snyder L. The lit gene product which blocks bacteriophage T4 late gene expression is a membrane protein encoded by a cryptic DNA element, e14. J Bacteriol. 1988 May;170(5):2056–2062. doi: 10.1128/jb.170.5.2056-2062.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Leigh J. A., Lee C. C. Characterization of polysaccharides of Rhizobium meliloti exo mutants that form ineffective nodules. J Bacteriol. 1988 Aug;170(8):3327–3332. doi: 10.1128/jb.170.8.3327-3332.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Leigh J. A., Signer E. R., Walker G. C. Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6231–6235. doi: 10.1073/pnas.82.18.6231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nikaido H., Nakae T. The outer membrane of Gram-negative bacteria. Adv Microb Physiol. 1979;20:163–250. doi: 10.1016/s0065-2911(08)60208-8. [DOI] [PubMed] [Google Scholar]
  21. Orgambide G., Montrozier H., Servin P., Roussel J., Trigalet-Demery D., Trigalet A. High heterogeneity of the exopolysaccharides of Pseudomonas solanacearum strain GMI 1000 and the complete structure of the major polysaccharide. J Biol Chem. 1991 May 5;266(13):8312–8321. [PubMed] [Google Scholar]
  22. Puvanesarajah V., Schell F. M., Stacey G., Douglas C. J., Nester E. W. Role for 2-linked-beta-D-glucan in the virulence of Agrobacterium tumefaciens. J Bacteriol. 1985 Oct;164(1):102–106. doi: 10.1128/jb.164.1.102-106.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Staskawicz B., Dahlbeck D., Keen N., Napoli C. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J Bacteriol. 1987 Dec;169(12):5789–5794. doi: 10.1128/jb.169.12.5789-5794.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  25. Van Alfen N. K., McMillan B. D., Turner V., Hess W. M. Role of pit membranes in macromolecule-induced wilt of plants. Plant Physiol. 1983 Dec;73(4):1020–1023. doi: 10.1104/pp.73.4.1020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Verma N., Reeves P. Identification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae. J Bacteriol. 1989 Oct;171(10):5694–5701. doi: 10.1128/jb.171.10.5694-5701.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Xu P. L., Iwata M., Leong S., Sequeira L. Highly virulent strains of Pseudomonas solanacearum that are defective in extracellular-polysaccharide production. J Bacteriol. 1990 Jul;172(7):3946–3951. doi: 10.1128/jb.172.7.3946-3951.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Xu P. L., Leong S., Sequeira L. Molecular cloning of genes that specify virulence in Pseudomonas solanacearum. J Bacteriol. 1988 Feb;170(2):617–622. doi: 10.1128/jb.170.2.617-622.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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