U snRNP assembly in yeast involves the La protein. Xue Dahai, Rubinson Douglas A., Pannone Barbara K., Yoo Christopher J., Wolin Sandra L. The EMBO Journal. 2000;19:1650–1660. doi: 10.1093/emboj/19.7.1650.
The publishers apologize for the poor printing quality of Figure 5. The figure is reprinted below.
Fig. 5. Analysis of U RNA levels during growth of smd1-1 cells at 16°C. (A) The growth of wild-type cells, SMD1 lhp1::LEU2 cells and smd1-1 cells containing either no LHP1 (smd1-1 lhp1::LEU2), chromosomal LHP1 (smd1-1 LHP1) or plasmid LHP1 [smd1-1 (pLHP1)] is compared. Cells were grown to OD = 0.3 at 30°C and switched to 16°C at time 0. (B and C) At intervals after the switch to 16°C, RNA was extracted from the strains and subjected to Northern analysis. The blot was probed to detect U1 and U2 RNAs (B) or U4, U5 and U6 RNAs (C).