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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1922 Mar 31;35(4):553–559. doi: 10.1084/jem.35.4.553

EXPERIMENTAL STUDIES OF THE NASOPHARYNGEAL SECRETIONS FROM INFLUENZA PATIENTS

VII. SEROLOGICAL REACTIONS.

Peter K Olitsky 1, Frederick L Gates 1
PMCID: PMC2128118  PMID: 19868628

Abstract

Cultivation of Bacterium pneumosintes in the collodion sac dialysate of a tissue medium produces an antigen suitable for serological tests. Injection of dialysate cultures of Bacterium pneumosintes into rabbits results in the production of antibodies demonstrable by agglutination, precipitation, complement fixation, and phagocytic reactions. Four strains of Bacterium pneumosintes, three from the first epidemic influenzal wave (1918–19) and one from the second (1920), show identical antigenic characters. The blood serum of rabbits experimentally injected with the glycerolated active material of rabbit passages contains specific agglutinins for Bacterium pneumosintes, whereas normal rabbit serum does not.

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Selected References

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  1. Olitsky P. K., Gates F. L. EXPERIMENTAL STUDIES OF THE NASOPHARYNGEAL SECRETIONS FROM INFLUENZA PATIENTS : I. TRANSMISSION EXPERIMENTS WITH NASOPHARYNGEAL WASHINGS. J Exp Med. 1921 Jan 31;33(2):125–145. doi: 10.1084/jem.33.2.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Olitsky P. K., Gates F. L. EXPERIMENTAL STUDIES OF THE NASOPHARYNGEAL SECRETIONS FROM INFLUENZA PATIENTS : IV. ANAEROBIC CULTIVATION. J Exp Med. 1921 May 31;33(6):713–729. doi: 10.1084/jem.33.6.713. [DOI] [PMC free article] [PubMed] [Google Scholar]

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