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. 1986 Jul;167(1):148–152. doi: 10.1128/jb.167.1.148-152.1986

Predominance of gluconate formation from glucose during germination of Bacillus megaterium QM B1551 spores.

M Otani, N Ihara, C Umezawa, K Sano
PMCID: PMC212853  PMID: 3013833

Abstract

Metabolic pathways of glucose during germination of Bacillus megaterium QM B1551 spores were studied by using specifically labeled glucose and gluconate. The Embden-Meyerhof pathway, the pentose cycle, and the direct oxidation route of glucose to gluconate (the gluconate pathway) were all operative at this stage; among those, gluconate accumulation was most predominant, especially in the early stage. Potassium fluoride, an enolase inhibitor, abolished the catabolism by the Embden-Meyerhof pathway totally without affecting gluconate accumulation. Under these conditions glucose was exclusively oxidized to gluconate. Gluconate thus accumulated could be metabolized further via phosphorylation by gluconate kinase. Remarkable gluconate accumulation was also demonstrated in several other spores requiring alanine as an effective germinant. NADH formed by the direct glucose oxidation may serve as a initial ATP source to phosphorylate glucose in germinating spores.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahnweg G., Douthit H. A. Cytochrome pigments in spores of Bacillus cereus T. J Bacteriol. 1975 Feb;121(2):737–739. doi: 10.1128/jb.121.2.737-739.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark M. G., Kneer N. M., Bosch A. L., Lardy H. A. The fructose 1,6-diphosphatase-phosphofructokinase substrate cycle. A site of regulation of hepatic gluconeogenesis by glucagon. J Biol Chem. 1974 Sep 25;249(18):5695–5703. [PubMed] [Google Scholar]
  3. DOI R., HALVORSON H., CHURCH B. Intermediate metabolism of aerobic spores. III. The mechanism of glucose and hexose phosphate oxidation in extracts of Bacillus cereus spores. J Bacteriol. 1959 Jan;77(1):43–54. doi: 10.1128/jb.77.1.43-54.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dills S. S., Vary J. C. An evaluation of respiration chain-associated functions during initiation of germination of Bacillus megaterium spores. Biochim Biophys Acta. 1978 Jul 3;541(3):301–311. doi: 10.1016/0304-4165(78)90190-3. [DOI] [PubMed] [Google Scholar]
  5. GOLDMAN M., BLUMENTHAL H. J. PATHWAYS OF GLUCOSE CATABOLISM IN BACILLUS CEREUS. J Bacteriol. 1964 Feb;87:377–386. doi: 10.1128/jb.87.2.377-386.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HACHISUKA Y., ASANO N., KANEKO M., KANBE T. Evolution of respiratory enzyme system during germination of Bacillus subtilis. Science. 1956 Jul 27;124(3213):174–175. doi: 10.1126/science.124.3213.174. [DOI] [PubMed] [Google Scholar]
  7. HACHISUKA Y., ASANO N., SUGAI K. Studies on spore germination. III. Development of enzymes concerning glucose oxidation during spore germination of Bacillus subtilis. Jpn J Microbiol. 1958 Jan;2(1):79–88. [PubMed] [Google Scholar]
  8. Maruyama T., Otani M., Sano K., Umezawa C. Glucose catabolism during germination of Bacillus megaterium spores. J Bacteriol. 1980 Mar;141(3):1443–1446. doi: 10.1128/jb.141.3.1443-1446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nelson D. L., Kornberg A. Biochemical studies of bacterial sporulation and germination. XX. Phosphate metabolism during germination. J Biol Chem. 1970 Mar 10;245(5):1146–1155. [PubMed] [Google Scholar]
  10. Nihashi J., Fujita Y. Catabolite repression of inositol dehydrogenase and gluconate kinase syntheses in Bacillus subtilis. Biochim Biophys Acta. 1984 Mar 22;798(1):88–95. doi: 10.1016/0304-4165(84)90014-x. [DOI] [PubMed] [Google Scholar]
  11. Prasad C., Diesterhaft M., Freese E. Initiation of spore germination in glycolytic mutants of Bacillus subtilis. J Bacteriol. 1972 Apr;110(1):321–328. doi: 10.1128/jb.110.1.321-328.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ROSE I. A., O'CONNELL E. L. Intramolecular hydrogen transfer in the phosphoglucose isomerase reaction. J Biol Chem. 1961 Dec;236:3086–3092. [PubMed] [Google Scholar]
  13. Racine F. M., Dills S. S., Vary J. C. Glucose-triggered germination of Bacillus megaterium spores. J Bacteriol. 1979 May;138(2):442–445. doi: 10.1128/jb.138.2.442-445.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Racine F. M., Vary J. C. Isolation and properties of membranes from Bacillus megaterium spores. J Bacteriol. 1980 Sep;143(3):1208–1214. doi: 10.1128/jb.143.3.1208-1214.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Setlow B., Setlow P. Levels of acetyl coenzyme A, reduced and oxidized coenzyme A, and coenzyme A in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of Bacillus megaterium. J Bacteriol. 1977 Nov;132(2):444–452. doi: 10.1128/jb.132.2.444-452.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Setlow B., Setlow P. Levels of oxidized and reduced pyridine nucleotides in dormant spores and during growth, sporulation, and spore germination of Bacillus megaterium. J Bacteriol. 1977 Feb;129(2):857–865. doi: 10.1128/jb.129.2.857-865.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Setlow B., Shay L. K., Vary J. C., Setlow P. Production of large amounts of acetate during germination of Bacillus megaterium spores in the absence of exogenous carbon sources. J Bacteriol. 1977 Nov;132(2):744–746. doi: 10.1128/jb.132.2.744-746.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores. J Biol Chem. 1970 Jul 25;245(14):3637–3644. [PubMed] [Google Scholar]
  19. Shay L. K., Vary J. C. Biochemical studies on glucose initiated germination in Bacillus megaterium. Biochim Biophys Acta. 1978 Jan 18;538(2):284–292. doi: 10.1016/0304-4165(78)90356-2. [DOI] [PubMed] [Google Scholar]
  20. Singh R. P., Setlow P. Enolase from spores and cells of Bacillus megaterium: two-step purification of the enzyme and some of its properties. J Bacteriol. 1978 Apr;134(1):353–355. doi: 10.1128/jb.134.1.353-355.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tochikubo K. Changes in terminal respiratory pathways of Bacillus subtilis during germination, outgrowth and vegetative growth. J Bacteriol. 1971 Nov;108(2):652–661. doi: 10.1128/jb.108.2.652-661.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wilkinson B. J., Ellar D. J. Morphogenesis of the membrane-bound electron-transport system in sporulating Bacillus megaterium KM. Eur J Biochem. 1975 Jun 16;55(1):131–139. doi: 10.1111/j.1432-1033.1975.tb02145.x. [DOI] [PubMed] [Google Scholar]

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