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. 1986 Jul;167(1):251–256. doi: 10.1128/jb.167.1.251-256.1986

Expression of the unassembled capsid protein during infection of Shigella sonnei by bacteriophage T7 results in DNA damage that is repairable by bacteriophage T3, but not T7, DNA ligase.

P J Beck, J P Condreay, I J Molineux
PMCID: PMC212868  PMID: 3522545

Abstract

The abortive infection of bacteriophage T7 in Shigella sonnei D2 371-48 is characterized by a premature inhibition of phage DNA replication and nucleolytic breakdown of all phage DNA. Mutations in T7 gene 10 which are recessive to the presence of the wild-type allele can alleviate the restriction of phage growth. Phage T3 productively infects S. sonnei D2 371-48, as does a T7-T3 hybrid phage that contains, in particular, a gene 10 of T7 origin. It is the presence of T3 DNA ligase that allows phage growth on S. sonnei D2 371-48, and this enzyme can also rescue wild-type T7 from the abortive infection. T7+ is therefore functionally ligase deficient during the infection of S. sonnei D2 371-48; this deficiency is a result of the expression of the phage capsid protein, but it is independent of the assembly of the protein into a procapsid or other morphogenetic structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Center M. S. Bacteriophage T7-induced endonuclease II. Purification and properties of the enzyme. J Biol Chem. 1972 Jan 10;247(1):146–156. [PubMed] [Google Scholar]
  2. Dunn J. J., Studier F. W. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol. 1983 Jun 5;166(4):477–535. doi: 10.1016/s0022-2836(83)80282-4. [DOI] [PubMed] [Google Scholar]
  3. Dunn J. J., Studier F. W. Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4. J Mol Biol. 1981 Jun 5;148(4):303–330. doi: 10.1016/0022-2836(81)90178-9. [DOI] [PubMed] [Google Scholar]
  4. Georgopoulos C. P., Hendrix R. W., Casjens S. R., Kaiser A. D. Host participation in bacteriophage lambda head assembly. J Mol Biol. 1973 May 5;76(1):45–60. doi: 10.1016/0022-2836(73)90080-6. [DOI] [PubMed] [Google Scholar]
  5. Gottesman M. M., Hicks M. L., Gellert M. Genetics and function of DNA ligase in Escherichia coli. J Mol Biol. 1973 Jul 15;77(4):531–547. doi: 10.1016/0022-2836(73)90221-0. [DOI] [PubMed] [Google Scholar]
  6. Hausmann R. Bacteriophage T7 genetics. Curr Top Microbiol Immunol. 1976;75:77–110. doi: 10.1007/978-3-642-66530-1_3. [DOI] [PubMed] [Google Scholar]
  7. Hausmann R., Gomez B., Moody B. Physiological and genetic aspects of abortive infection of a Shigella sonnei strain by coliphage T7. J Virol. 1968 Apr;2(4):335–345. doi: 10.1128/jvi.2.4.335-345.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hausmann R. Sedimentation analysis of phage T7-directed DNA synthesized in the presence of a dominant conditional lethal phage gene. Biochem Biophys Res Commun. 1968 May 23;31(4):609–615. doi: 10.1016/0006-291x(68)90522-6. [DOI] [PubMed] [Google Scholar]
  9. Hausmann R. The genetics of T-odd phages. Annu Rev Microbiol. 1973;27:51–67. doi: 10.1146/annurev.mi.27.100173.000411. [DOI] [PubMed] [Google Scholar]
  10. Hyman R. W., Brunovskis I., Summers W. C. A biochemical comparison of the related bacteriophages T7, phiI, phiII, W31, H, and T3. Virology. 1974 Jan;57(1):189–206. doi: 10.1016/0042-6822(74)90120-2. [DOI] [PubMed] [Google Scholar]
  11. Krüger D. H., Schroeder C. Bacteriophage T3 and bacteriophage T7 virus-host cell interactions. Microbiol Rev. 1981 Mar;45(1):9–51. doi: 10.1128/mr.45.1.9-51.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Manne V., Rao V. B., Black L. W. A bacteriophage T4 DNA packaging related DNA-dependent ATPase-endonuclease. J Biol Chem. 1982 Nov 25;257(22):13223–13232. [PubMed] [Google Scholar]
  13. Mertens H., Hausmann R. Coliphage BA14: a new relative of phage T7. J Gen Virol. 1982 Oct;62(Pt 2):331–341. doi: 10.1099/0022-1317-62-2-331. [DOI] [PubMed] [Google Scholar]
  14. Molineux I. J., Mooney P. Q., Spence J. L. Recombinants between bacteriophages T7 and T3 which productively infect F-plasmid-containing strains of Escherichia coli. J Virol. 1983 Jun;46(3):881–894. doi: 10.1128/jvi.46.3.881-894.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Molineux I. J., Spence J. L. Virus-plasmid interactions: mutants of bacteriophage T3 that abortively infect plasmid F-containing (F+) strains of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1465–1469. doi: 10.1073/pnas.81.5.1465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pacumbaba R. P., Center M. S. Studies on an endonuclease activity associated with bacteriophage T7 DNA-membrane complex. J Virol. 1974 Dec;14(6):1380–1387. doi: 10.1128/jvi.14.6.1380-1387.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roeder G. S., Sadowski P. D. Bacteriophage T7 morphogenesis: phage-related particles in cells infected with wild-type and mutant T7 phage. Virology. 1977 Jan;76(1):263–285. doi: 10.1016/0042-6822(77)90302-6. [DOI] [PubMed] [Google Scholar]
  18. Sadowski P. D. An endodeoxyribonuclease induced after infection with phage T7 bearing amber mutations in genes 3, 5, and 6. Can J Biochem. 1972 Sep;50(9):1016–1023. doi: 10.1139/o72-140. [DOI] [PubMed] [Google Scholar]
  19. Serwer P. Internal proteins of bacteriophage T7. J Mol Biol. 1976 Nov 5;107(3):271–291. doi: 10.1016/s0022-2836(76)80005-8. [DOI] [PubMed] [Google Scholar]
  20. Spence J. L., Mooney P. Q., Molineux I. J. Physiological properties of a T7-T3 recombinant bacteriophage that productively infects strains of Escherichia coli that harbor the F plasmid. J Virol. 1983 Jun;46(3):895–900. doi: 10.1128/jvi.46.3.895-900.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sternberg N. Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). II. The propagation of phage lambda. J Mol Biol. 1973 May 5;76(1):25–44. doi: 10.1016/0022-2836(73)90079-x. [DOI] [PubMed] [Google Scholar]
  22. Studier F. W. Genetic analysis of non-essential bacteriophage T7 genes. J Mol Biol. 1973 Sep 15;79(2):227–236. doi: 10.1016/0022-2836(73)90002-8. [DOI] [PubMed] [Google Scholar]
  23. Studier F. W., Movva N. R. SAMase gene of bacteriophage T3 is responsible for overcoming host restriction. J Virol. 1976 Jul;19(1):136–145. doi: 10.1128/jvi.19.1.136-145.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Studier F. W. Relationships among different strains of T7 and among T7-related bacteriophages. Virology. 1979 May;95(1):70–84. doi: 10.1016/0042-6822(79)90402-1. [DOI] [PubMed] [Google Scholar]
  25. Vetter D., Roberts L., Jackowski J. B., Sadowski P. D. Location of the ss--mutation of bacteriophage T7 in genes 10, the structural gene for the major capsid protein. J Virol. 1977 Jun;22(3):694–701. doi: 10.1128/jvi.22.3.694-701.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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