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. 1986 Jul;167(1):420–422. doi: 10.1128/jb.167.1.420-422.1986

Mutant of Salmonella typhimurium LT2 deficient in DNA adenine methylation.

L J Ritchie, R M Hall, D M Podger
PMCID: PMC212898  PMID: 3522556

Abstract

A mutant of Salmonella typhimurium LT2 deficient in methylation of the adenine residues in the sequence 5'-GATC-3' was isolated. The mutation (dam-1) was linked to the cysG locus, and the properties of the mutant were similar to those of Escherichia coli dam mutants. Reversion of the hisC3076 frameshift marker by 9-aminoacridine was substantially enhanced by the dam-1 mutation, implying a direct role for adenine methylation in the prevention of frameshift mutation induction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames B. N., Mccann J., Yamasaki E. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res. 1975 Dec;31(6):347–364. doi: 10.1016/0165-1161(75)90046-1. [DOI] [PubMed] [Google Scholar]
  2. Brooks J. E., Blumenthal R. M., Gingeras T. R. The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. Nucleic Acids Res. 1983 Feb 11;11(3):837–851. doi: 10.1093/nar/11.3.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Glickman B. W., Radman M. Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063–1067. doi: 10.1073/pnas.77.2.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glickman B., van den Elsen P., Radman M. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance. Mol Gen Genet. 1978 Jul 25;163(3):307–312. doi: 10.1007/BF00271960. [DOI] [PubMed] [Google Scholar]
  5. Lederberg E. M., Cohen S. N. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974 Sep;119(3):1072–1074. doi: 10.1128/jb.119.3.1072-1074.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Marinus M. G. Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol Gen Genet. 1973 Dec 14;127(1):47–55. doi: 10.1007/BF00267782. [DOI] [PubMed] [Google Scholar]
  7. Mohn G. R., Kerklaan P. R., van Zeeland A. A., Ellenberger J., Baan R. A., Lohman P. H., Pons F. W. Methodologies for the determination of various genetic effects in permeable strains of E. coli K-12 differing in DNA repair capacity. Quantification of DNA adduct formation, experiments with organ homogenates and hepatocytes, and animal-mediated assays. Mutat Res. 1984 Feb;125(2):153–184. doi: 10.1016/0027-5107(84)90067-8. [DOI] [PubMed] [Google Scholar]
  8. Podger D. M., Hall R. M. Induction of SOS functions is not required for recA+-dependent mutagenicity of 9-aminoacridine in Salmonella typhimurium strain trpE8. Mutat Res. 1984 Mar-Apr;131(3-4):115–121. doi: 10.1016/0167-8817(84)90050-6. [DOI] [PubMed] [Google Scholar]
  9. Skopek T. R., Hutchinson F. Frameshift mutagenesis of lambda prophage by 9-aminoacridine, proflavin and ICR-191. Mol Gen Genet. 1984;195(3):418–423. doi: 10.1007/BF00341442. [DOI] [PubMed] [Google Scholar]
  10. Sternberg N. Evidence that adenine methylation influences DNA-protein interactions in Escherichia coli. J Bacteriol. 1985 Oct;164(1):490–493. doi: 10.1128/jb.164.1.490-493.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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