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. 1984 Aug;93(1):43–49. doi: 10.1017/s0022172400060915

Stability of aminoglycoside resistance in vitro in gentamicin-resistant Staphylococcus aureus.

S Dixson, W Brumfitt, J M Hamilton-Miller
PMCID: PMC2129279  PMID: 6747285

Abstract

Stability of aminoglycoside resistance has been investigated in 20 strains of Staphylococcus aureus resistant to gentamicin (16 strains were also resistant to methicillin). In view of previous reports that incubation at elevated temperatures can hasten the loss of unstable antibiotic resistance, we passaged strains daily in a liquid medium for 24 days at 43 degrees C. The nine strains which were resistant to neomycin kept their aminoglycoside resistance virtually intact, whereas most of the other 11 strains (sensitive to neomycin) lost almost all their resistance to gentamicin and kanamycin after 5 days. It thus appears that the stability of aminoglycoside resistances in Staph. aureus is closely linked to the resistance of the strains to neomycin. This finding has important possible consequences in terms of the advisability of the clinical usage of preparations containing neomycin or framycetin for topical application and bowel sterilization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alder V. G., Gillespie W. A. Influence of neomycin sprays on the spread of resistant staphylococci. Lancet. 1967 Nov 18;2(7525):1062–1063. doi: 10.1016/s0140-6736(67)90337-6. [DOI] [PubMed] [Google Scholar]
  2. Ayliffe G. A. Stability of neomycin resistance in Staphylococcus aureus. J Clin Pathol. 1970 Feb;23(1):19–23. doi: 10.1136/jcp.23.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bint A. J., George R. H., Healing D. E., Wise R., Davies M. An outbreak of infection caused by a gentamicin-resistant Staphylococcus aureus. J Clin Pathol. 1977 Feb;30(2):165–167. doi: 10.1136/jcp.30.2.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crossley K., Loesch D., Landesman B., Mead K., Chern M., Strate R. An outbreak of infections caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides. I. Clinical studies. J Infect Dis. 1979 Mar;139(3):273–279. doi: 10.1093/infdis/139.3.273. [DOI] [PubMed] [Google Scholar]
  5. FAIRBROTHER R. W., PARKER L., EATON B. R. The stability of penicillinase-producting strains of Staphylococcus aureus. J Gen Microbiol. 1954 Apr;10(2):309–316. doi: 10.1099/00221287-10-2-309. [DOI] [PubMed] [Google Scholar]
  6. Gentamicin-resistant staphylococci. Lancet. 1981 Sep 26;2(8248):698–699. [PubMed] [Google Scholar]
  7. Giamarellou H., Papapetropoulou M., Daikos G. K. 'Methicillin resistant' Staphylococcus aureus infections during 1978-79: clinical and bacteriologic observations. J Antimicrob Chemother. 1981 Jun;7(6):649–655. doi: 10.1093/jac/7.6.649. [DOI] [PubMed] [Google Scholar]
  8. Hone R., Cafferkey M., Keane C. T., Harte-Barry M., Moorhouse E., Carroll R., Martin F., Ruddy R. Bacteraemia in Dublin due to gentamicin-resistant Staphylococcus aureus. J Hosp Infect. 1981 Jun;2(2):119–126. doi: 10.1016/0195-6701(81)90020-7. [DOI] [PubMed] [Google Scholar]
  9. LOWBURY E. J., BABB J. R., BROWN V. L., COLLINS B. J. NEOMYCIN-RESISTANT STAPHYLOCOCCUS AUREUS IN A BURNS UNIT. J Hyg (Lond) 1964 Jun;62:221–228. doi: 10.1017/s0022172400039942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Linnemann C. C., Jr, Mason M., Moore P., Korfhagen T. R., Staneck J. L. Methicillin-resistant Staphylococcus aureus: experience in a general hospital over four years. Am J Epidemiol. 1982 Jun;115(6):941–950. doi: 10.1093/oxfordjournals.aje.a113381. [DOI] [PubMed] [Google Scholar]
  11. Lyon B. R., May J. W., Skurray R. A. Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1983 Jun;23(6):817–826. doi: 10.1128/aac.23.6.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MAY J. W., HOUGHTON R. H., PERRET C. J. THE EFFECT OF GROWTH AT ELEVATED TEMPERATURES ON SOME HERITABLE PROPERTIES OF STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1964 Nov;37:157–169. doi: 10.1099/00221287-37-2-157. [DOI] [PubMed] [Google Scholar]
  13. Naidoo J., Noble W. C. Acquisition of antibiotic resistance by Staphylococcus aureus in skin patients. J Clin Pathol. 1978 Dec;31(12):1187–1192. doi: 10.1136/jcp.31.12.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Naidoo J., Noble W. C., Weissmann A., Dyke K. G. Gentamicin-resistant staphylococci: genetics of an outbreak in a dermatology department. J Hyg (Lond) 1983 Aug;91(1):7–16. doi: 10.1017/s0022172400059970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pavillard R., Harvey K., Douglas D., Hewstone A., Andrew J., Collopy B., Asche V., Carson P., Davidson A., Gilbert G. Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Med J Aust. 1982 May 29;1(11):451–454. [PubMed] [Google Scholar]
  16. Peacock J. E., Jr, Marsik F. J., Wenzel R. P. Methicillin-resistant Staphylococcus aureus: introduction and spread within a hospital. Ann Intern Med. 1980 Oct;93(4):526–532. doi: 10.7326/0003-4819-93-4-526. [DOI] [PubMed] [Google Scholar]
  17. Porthouse A., Brown D. F., Smith R. G., Rogers T. Gentamicin resistance in Staphylococcus aureus. Lancet. 1976 Jan 3;1(7949):20–21. doi: 10.1016/s0140-6736(76)92912-3. [DOI] [PubMed] [Google Scholar]
  18. Price E. H., Brain A., Dickson J. A. An outbreak of infection with a gentamicin and methicillin-resistant Staphylococcus aureus in a neonatal unit. J Hosp Infect. 1980 Sep;1(3):221–228. doi: 10.1016/0195-6701(80)90059-6. [DOI] [PubMed] [Google Scholar]
  19. ROUNTREE P. M., BEARD M. A. THE SPREAD OF NEOMYCIN-RESISTANT STAPHYLOCOCCI IN A HOSPITAL. Med J Aust. 1965 Apr 3;1(14):498–502. doi: 10.5694/j.1326-5377.1965.tb71863.x. [DOI] [PubMed] [Google Scholar]
  20. Rosendal K., Bang J., Rosdahl V. T. Gentamicin-resistant Staphylococcus aureus strains isolated in Denmark in 1979. Acta Pathol Microbiol Scand B. 1981 Jun;89(3):185–191. doi: 10.1111/j.1699-0463.1981.tb00174_89b.x. [DOI] [PubMed] [Google Scholar]
  21. Shannon K., Phillips I. Mechanisms of resistance to aminoglycosides in clinical isolates. J Antimicrob Chemother. 1982 Feb;9(2):91–102. doi: 10.1093/jac/9.2.91. [DOI] [PubMed] [Google Scholar]
  22. Shanson D. C., Kensit J. C., Duke R. Outbreak of hospital infection with a strain of Staphylococcus aureus resistant to gentamicin and methicillin. Lancet. 1976 Dec 18;2(7999):1347–1348. doi: 10.1016/s0140-6736(76)91986-3. [DOI] [PubMed] [Google Scholar]
  23. Spitzy K. H., Rotter M. Resistente Staphylokokken neuerlich im Vormarsch? Infection. 1979;7 (Suppl 2):S220–S224. doi: 10.1007/BF01641127. [DOI] [PubMed] [Google Scholar]
  24. Townsend D. E., Grubb W. B., Ashdown N. Gentamicin resistance in methicillin-resistant Staphylococcus aureus. Pathology. 1983 Apr;15(2):169–174. doi: 10.3109/00313028309084707. [DOI] [PubMed] [Google Scholar]
  25. Wyatt T. D., Ferguson W. P., Wilson T. S., McCormick E. Gentamicin resistant Staphylococcus aureus associated with the use of topical gentamicin. J Antimicrob Chemother. 1977 May;3(3):213–217. doi: 10.1093/jac/3.3.213. [DOI] [PubMed] [Google Scholar]
  26. Yourassowsky E., van der Linden M. P., Lismont M. J., Crokaert F. Combination of minocycline and rifampicin against methicillin- and gentamicin-resistant Staphylococcus aureus. J Clin Pathol. 1981 May;34(5):559–563. doi: 10.1136/jcp.34.5.559. [DOI] [PMC free article] [PubMed] [Google Scholar]

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