Abstract
The resistance of spores of B. subtilis var. niger produced in liquid synthetic medium and exposed to ethylene oxide on a nylon surface, has been shown to the almost identical to that for spores produced on a traditional solidified complex medium with exposure to the sterilant on aluminium foil. The use of short lengths of nylon tube as carriers allowed easy production and handling, with self-protection of the spore-bearing surface. Addition of a dye provided visual evidence of inoculation without affecting resistance to ethylene oxide. Such a monitor is suitable for use as a standardized biological challenge in routine ethylene oxide sterilization cycles.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beeby M. M., Whitehouse C. E. A bacterial spore test piece for the control of ethylene oxide sterilization. J Appl Bacteriol. 1965 Dec;28(3):349–360. doi: 10.1111/j.1365-2672.1965.tb02163.x. [DOI] [PubMed] [Google Scholar]
- Buono F., Testa R., Lundgren D. G. Physiology of growth and sporulation in Bacillus cereus. I. Effect of glutamic and other amino acids. J Bacteriol. 1966 Jun;91(6):2291–2299. doi: 10.1128/jb.91.6.2291-2299.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DONNELLAN J. E., Jr, NAGS E. H., LEVINSON H. S. CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS. J Bacteriol. 1964 Feb;87:332–336. doi: 10.1128/jb.87.2.332-336.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dadd A. H., Daley G. M. Resistance of micro-organisms to inactivation by gaseous ethylene oxide. J Appl Bacteriol. 1980 Aug;49(1):89–101. doi: 10.1111/j.1365-2672.1980.tb01046.x. [DOI] [PubMed] [Google Scholar]
- Hodges N. A., Melling J., Parker S. J. A comparison of chemically defined and complex media for the production of Bacillus subtilis spores having reproducible resistance and germination characteristics. J Pharm Pharmacol. 1980 Feb;32(2):126–130. doi: 10.1111/j.2042-7158.1980.tb12867.x. [DOI] [PubMed] [Google Scholar]
- ROWLEY D. B., NEWCOMB H. R. RADIOSENSITIVITY OF SEVERAL DEHYDROGENASES AND TRANSAMINASES DURING SPOROGENESIS OF BACILLUS SUBTILIS. J Bacteriol. 1964 Mar;87:701–709. doi: 10.1128/jb.87.3.701-709.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]