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. 1984 Jun;92(3):377–384. doi: 10.1017/s0022172400064597

Distribution of multilocus genotypes of Escherichia coli within and between host families.

D A Caugant, B R Levin, R K Selander
PMCID: PMC2129322  PMID: 6376625

Abstract

Isolates from the intestinal Escherichia coli flora of 28 members of five families (including parents, children, and household pets) in Amherst, Massachusetts, and Rochester, New York, were characterized by the electrophoretic mobilities of 12 enzymes to estimate the extent of sharing of strains among associated and unassociated hosts. Among the 655 isolates examined, 60 different combinations of electromorphs (electrophoretic types or ETs), each representing a distinctive multilocus genotype, were identified, of which 85% were recovered from only a single individual. On average, 11% of the ETs isolated from the same family were shared by two or more members; 4.9% of ETs were shared among members of unassociated families living in the same city; and only 2% were shared by families in different cities. All three ETs that were recovered from multiple hosts in the present study are widespread clones that have been isolated from many other host individuals in North America and Sweden.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R. P. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun. 1983 Jan;39(1):315–335. doi: 10.1128/iai.39.1.315-335.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bettelheim K. A., Breadon A., Faiers M. C., O'Farrell S. M., Shooter R. A. The origin of O serotypes of Escherichia coli in babies after normal delivery. J Hyg (Lond) 1974 Feb;72(1):67–70. doi: 10.1017/s0022172400023226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bettelheim K. A., Cooke E. M., O'Farrell S., Shooter R. A. The effect of diet on intestinal Escherichia coli. J Hyg (Lond) 1977 Aug;79(1):43–45. doi: 10.1017/s0022172400052839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bettelheim K. A., Teoh-Chan C. H., Chandler M. E., O'Farrell S. M., Rahamim L., Shaw E. J., Shooter R. A. Spread of Escherichia coli colonizing newborn babies and their mothers. J Hyg (Lond) 1974 Dec;73(3):383–387. [PMC free article] [PubMed] [Google Scholar]
  5. Bettelheim K. A. The sources of "OH" serotypes of Escherichia coli. J Hyg (Lond) 1978 Feb;80(1):83–113. doi: 10.1017/s0022172400053420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Caugant D. A., Levin B. R., Lidin-Janson G., Whittam T. S., Svanborg Edén C., Selander R. K. Genetic diversity and relationships among strains of Escherichia coli in the intestine and those causing urinary tract infections. Prog Allergy. 1983;33:203–227. doi: 10.1159/000318331. [DOI] [PubMed] [Google Scholar]
  7. Caugant D. A., Levin B. R., Selander R. K. Genetic diversity and temporal variation in the E. coli population of a human host. Genetics. 1981 Jul;98(3):467–490. doi: 10.1093/genetics/98.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cooke E. M., Ewins S., Shooter R. A. Changing faecal population of Escherichia coli in hospital medical patients. Br Med J. 1969 Dec 6;4(5683):593–595. doi: 10.1136/bmj.4.5683.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kusecek B., Wloch H., Mercer A., Vaisänen V., Pluschke G., Korhonen T., Achtman M. Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli. Infect Immun. 1984 Jan;43(1):368–379. doi: 10.1128/iai.43.1.368-379.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ochman H., Selander R. K. Evidence for clonal population structure in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jan;81(1):198–201. doi: 10.1073/pnas.81.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ochman H., Whittam T. S., Caugant D. A., Selander R. K. Enzyme polymorphism and genetic population structure in Escherichia coli and Shigella. J Gen Microbiol. 1983 Sep;129(9):2715–2726. doi: 10.1099/00221287-129-9-2715. [DOI] [PubMed] [Google Scholar]
  12. Orskov F., Orskov I., Evans D. J., Jr, Sack R. B., Sack D. A., Wadström T. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med Microbiol Immunol. 1976 Jun 1;162(2):73–80. doi: 10.1007/BF02121318. [DOI] [PubMed] [Google Scholar]
  13. Orskov I., Orskov F., Jann B., Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977 Sep;41(3):667–710. doi: 10.1128/br.41.3.667-710.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rowe B., Taylor J., Bettelheim K. A. An investigation of traveller's diarrhoea. Lancet. 1970 Jan 3;1(7636):1–5. doi: 10.1016/s0140-6736(70)90520-9. [DOI] [PubMed] [Google Scholar]
  15. SEARS H. J., BROWNLEE I. Further observations on the persistence of individual strains of Escherichia coli in the intestinal tract of man. J Bacteriol. 1952 Jan;63(1):47–57. doi: 10.1128/jb.63.1.47-57.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Selander R. K., Levin B. R. Genetic diversity and structure in Escherichia coli populations. Science. 1980 Oct 31;210(4469):545–547. doi: 10.1126/science.6999623. [DOI] [PubMed] [Google Scholar]
  17. Shooter R. A., Bettleheim K. A., Lennox-King S. M., O'Farrell S. Escherichia coli serotypes in the faeces of healthy adults over a period of several months. J Hyg (Lond) 1977 Feb;78(1):95–98. doi: 10.1017/s0022172400055972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Silver R. P., Aaronson W., Sutton A., Schneerson R. Comparative analysis of plasmids and some metabolic characteristics of Escherichia coli K1 from diseased and healthy individuals. Infect Immun. 1980 Jul;29(1):200–206. doi: 10.1128/iai.29.1.200-206.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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