Abstract
A micro test for the detection of rubella IgG antibody by passive haemolysis in free solution (MIH) is presented. The technique is reproducible, sensitive, simple to perform and economical in the use of reagents. Identical results were obtained in all but one of 408 tests on serum specimens screened for rubella antibody at 15 i.u./ml by the MIH and haemagglutination inhibition (HI) techniques. The quantitative potential of the MIH test and the relative merits of the MIH, HI and radial haemolysis (RH) procedures for rubella screening are discussed.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Forger J. M., 3rd, Gilfillan R. F. Single-radial hemolysis as a cost-effective determinant of Rubella antibody status. J Clin Microbiol. 1979 Jan;9(1):115–119. doi: 10.1128/jcm.9.1.115-119.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mortimer P. P., Edwards J. M., Porter A. D., Tedder R. S., Mace J. E., Hutchinson A. Are many women immunized against rubella unnecessarily? J Hyg (Lond) 1981 Aug;87(1):131–138. doi: 10.1017/s0022172400069308. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORRBY E. Hemagglutination by measles virus. 4. A simple procedure for production of high potency antigen for hemagglutination-inhibition (HI) tests. Proc Soc Exp Biol Med. 1962 Dec;111:814–818. doi: 10.3181/00379727-111-27930. [DOI] [PubMed] [Google Scholar]
- Pattison J. R. Rubella antibody screening. J Hyg (Lond) 1982 Apr;88(2):149–153. doi: 10.1017/s0022172400070029. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schild G. C., Pereira M. S., Chakraverty P. Single-radial-hemolysis: a new method for the assay of antibody to influenza haemagglutinin. Applications for diagnosis and seroepidemiologic surveillance of influenza. Bull World Health Organ. 1975;52(1):43–50. [PMC free article] [PubMed] [Google Scholar]
- Skaug K., Orstavik I., Ulstrup J. C. Application of the passive haemolysis test for the determination of rubella virus antibodies. Acta Pathol Microbiol Scand B. 1975 Aug;83(4):367–372. doi: 10.1111/j.1699-0463.1975.tb00114.x. [DOI] [PubMed] [Google Scholar]
- Strannegård O., Grillner L., Lindberg I. M. Hemolysis-in-gel test for the demonstration of antibodies to rubella virus. J Clin Microbiol. 1975 Jun;1(6):491–494. doi: 10.1128/jcm.1.6.491-494.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
