Skip to main content
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1983 Oct;91(2):267–271. doi: 10.1017/s0022172400060277

Effect of stool storage at room temperature on salmonella isolation from faeces.

R W Harvey, T H Price
PMCID: PMC2129379  PMID: 6644012

Abstract

Stools, known to have contained salmonellas were cultured in three enrichment media on arrival at the laboratory. The three media were laboratory prepared selenite F, laboratory prepared Muller-Kauffmann tetrathionate and commercially obtained Oxoid Muller-Kauffmann tetrathionate (CM343). Where faecal samples were sufficiently large, they were stored at room temperature and re-examined using the same three enrichment broths. Whether fresh or stored stools were investigated, the laboratory prepared media were significantly more efficient than the commercial medium. In a straight comparison of the two laboratory prepared media, no significant difference in efficiency was evident with fresh stool samples. With stored specimens, however, Muller-Kauffmann tetrathionate was significantly better than selenite F. This finding might be relevant in examining samples delayed in the post.

Full text

PDF
267

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Edel W., Kampelmacher E. H. Salmonella isolation in nine European laboratories using a standardized technique. Bull World Health Organ. 1969;41(2):297–306. [PMC free article] [PubMed] [Google Scholar]
  2. HARVEY R. W. Choice of a selective medium for the routine isolation of members of the Salmonella group. Mon Bull Minist Health Public Health Lab Serv. 1956 Jun;15:118–124. [PubMed] [Google Scholar]
  3. HARVEY R. W., THOMSON S. Optimum temperature of incubation for isolation of Salmonellae. Mon Bull Minist Health Public Health Lab Serv. 1953 Jul;12:149–150. [PubMed] [Google Scholar]
  4. Harvey R. W., Price T. H. A comparisoin of the R25 modification of Rappaport s enrichment medium with strontium chloride B for salmonella isolation from sewage polluted natural water. J Hyg (Lond) 1982 Aug;89(1):111–117. doi: 10.1017/s0022172400070595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harvey R. W., Price T. H. Observations on pre-enrichment for isolating salmonellas from sewage polluted natural water using Muller-Kauffmann tetrathionate broth prepared with fresh and desiccated ox bile. J Appl Bacteriol. 1977 Aug;43(1):145–148. doi: 10.1111/j.1365-2672.1977.tb00733.x. [DOI] [PubMed] [Google Scholar]
  6. Harvey R. W., Price T. H. Quality control tests of two salmonella enrichment media using different inocula. J Hyg (Lond) 1975 Jun;74(3):375–384. doi: 10.1017/s0022172400046891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harvey R. W., Price T. H. Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios. J Hyg (Lond) 1980 Aug;85(1):125–128. doi: 10.1017/s0022172400027133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harvey R. W., Price T. H. Studies on the isolation of Salmonella dublin. J Hyg (Lond) 1975 Jun;74(3):369–374. doi: 10.1017/s002217240004688x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harvey R. W., Price T. H., Xirouchaki E. Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. J Hyg (Lond) 1979 Dec;83(3):451–460. doi: 10.1017/s0022172400026292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Iveson J. B., Mackay-Scollay E. M. Strontium chloride and strontium selenite enrichment broth media in the isolation of Salmonella. J Hyg (Lond) 1969 Sep;67(3):457–464. doi: 10.1017/s0022172400041875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCoy J. H. Trends in salmonella food poisoning in England and Wales 1941-72. J Hyg (Lond) 1975 Apr;74(2):271–282. doi: 10.1017/s0022172400024347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Price T. H. Isolation of Shigella sonnei by fluid media. J Hyg (Lond) 1976 Dec;77(3):341–348. doi: 10.1017/s0022172400055704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Read R. B., Jr, Reyes A. L. Variation in plating efficiency of Salmonellae on eight lots of brilliant green agar. Appl Microbiol. 1968 May;16(5):746–748. doi: 10.1128/am.16.5.746-748.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts D., Boag K., Hall M. L., Shipp C. R. The isolation of salmonellas from British pork sausages and sausage meat. J Hyg (Lond) 1975 Oct;75(2):173–184. doi: 10.1017/s0022172400047203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. THOMSON S. The numbers of pathogenic bacilli in faeces in intestinal diseases. J Hyg (Lond) 1955 Jun;53(2):217–224. doi: 10.1017/s0022172400000681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taylor W. I., Schelhart D. Isolation of shigellae. V. Comparison of enrichment broths with stools. Appl Microbiol. 1968 Sep;16(9):1383–1386. doi: 10.1128/am.16.9.1383-1386.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vassiliadis P., Pateraki E., Papadakis J., Trichopoulos D. Evaluation of the growth of salmonellae in Rappaport's broth and in Muller-Kauffmann's Tetrathionate broth. J Appl Bacteriol. 1974 Sep;37(3):411–418. doi: 10.1111/j.1365-2672.1974.tb00456.x. [DOI] [PubMed] [Google Scholar]
  18. van Schothorst M., van Leusden F. M., Jeunink J., de Dreu J. Studies on the multiplication of salmonellae in various enrichment media at different incubation temperatures. J Appl Bacteriol. 1977 Apr;42(2):157–163. doi: 10.1111/j.1365-2672.1977.tb00681.x. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES