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. 1984 Oct;93(2):317–323. doi: 10.1017/s0022172400064858

The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces.

D Baxby, N Blundell, C A Hart
PMCID: PMC2129424  PMID: 6209333

Abstract

Features are described of a new staining method for the detection of Cryptosporidium oocysts in faeces. Smears, fixed in acid-methanol, are stained with heated safranin and counterstained with methylene blue. Oocysts stain a vivid orange-pink and are easily recognized. The method is rapid and simple with little source of error. The method is more sensitive than currently recommended Ziehl-Neelson methods; of 26 cases of cryptosporidiosis diagnosed by the new method only 19 were detected by acid-fast and only 11 by acid-alcohol-fast methods. Oocysts can be stained by safranin-methylene blue after concentration by various methods, in paraffin-embedded material, and after storage for months at various temperatures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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