Abstract
Studies of biosynthetic pathways with purines as substrates showed that Methanococcus vannielii was capable of degrading xanthine to an extent that several of the carbon atoms were converted to CO2. Experiments to determine whether this catabolic activity could satisfy the entire nitrogen requirement for growth of M. vannielii showed that urate, guanine, xanthine, or hypoxanthine, but not adenine, could serve as the sole nitrogen source. The pyrimidines uracil and thymine, but not cytosine, were also degraded to serve as a source of nitrogen. Although urate was extensively degraded, it did not replace formate as the sole carbon source for growth of M. vannielii under the conditions imposed.
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Selected References
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- Barker H. A., Beck J. V. Clostridium acidi-uridi and Clostridium cylindrosporum, Organisms Fermenting Uric Acid and Some Other Purines. J Bacteriol. 1942 Mar;43(3):291–304. doi: 10.1128/jb.43.3.291-304.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones J. B., Stadtman T. C. Methanococcus vannielii: culture and effects of selenium and tungsten on growth. J Bacteriol. 1977 Jun;130(3):1404–1406. doi: 10.1128/jb.130.3.1404-1406.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- RABINOWITZ J. C., BARKER H. A. Purine fermentation by Clostridium cylindrosporum. I. Tracer experiments on the fermentation of guanine. J Biol Chem. 1956 Jan;218(1):147–160. [PubMed] [Google Scholar]
- RABINOWITZ J. C., BARKER H. A. Purine fermentation by Clostridium cylindrosporum. II. Purine transformations. J Biol Chem. 1956 Jan;218(1):161–173. [PubMed] [Google Scholar]
- Radin N. S., Barker H. A. Enzymatic Reactions in Purine Decomposition by Preparations of Clostridium Acidi-Urici. Proc Natl Acad Sci U S A. 1953 Dec;39(12):1196–1204. doi: 10.1073/pnas.39.12.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STADTMAN T. C., BARKER H. A. Studies on the methane fermentation. X. A new formate-decomposing bacterium, Methanococcus vannielii. J Bacteriol. 1951 Sep;62(3):269–280. doi: 10.1128/jb.62.3.269-280.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogels G. D., Van der Drift C. Degradation of purines and pyrimidines by microorganisms. Bacteriol Rev. 1976 Jun;40(2):403–468. doi: 10.1128/br.40.2.403-468.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHITELEY H. R., DOUGLAS H. C. The fermentation of purines by Micrococcus lactilyticus. J Bacteriol. 1951 May;61(5):605–616. doi: 10.1128/jb.61.5.605-616.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHITELEY H. R. The fermentation of purines by Micrococcus aerogenes. J Bacteriol. 1952 Feb;63(2):163–175. doi: 10.1128/jb.63.2.163-175.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]