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. 1985 Jun;94(3):263–268. doi: 10.1017/s0022172400061489

A study of enterotoxigenic Escherichia coli, serogroup 0126, by bacterial restriction endonuclease DNA analysis (BRENDA).

R B Marshall, P J Winter, A J Robinson, K A Bettelheim
PMCID: PMC2129484  PMID: 2989358

Abstract

Sixteen isolates of Escherichia coli were subjected to bacterial restriction endonuclease DNA analysis (BRENDA). Nine of these isolates were from an outbreak of human diarrhoea and produced stable toxin, the remaining seven were non-toxigenic strains from animal and human sources. The isolates from the outbreak produced indistinguishable DNA electrophoretic patterns in spite of their assignment to seven different H serotypes. Their BRENDA patterns were markedly different from the other isolates examined. These results support the epidemiological evidence that a single-strain outbreak had occurred, and they cast doubt on the value of H typing for this particular investigation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berry R. J., Bettelheim K. A., Gracey M. Studies on enterotoxigenic Escherichia coli isolated from persons without diarrhoea in Western Australia. J Hyg (Lond) 1983 Feb;90(1):99–106. doi: 10.1017/s0022172400063890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bettelheim K. A., Reeve K. G. An outbreak of gastroenteritis due to enteropathogenic Escherichia coli, which are also enterotoxigenic. N Z Med J. 1982 Apr 14;95(705):215–216. [PubMed] [Google Scholar]
  3. Bettelheim K. A., Wilson M. W., Shooter R. A., O'Farrell S. M. Studies on the enterotoxigenicity of environmental Escherichia coli, belonging to serotypes normally considered enterotoxigenic. J Hyg (Lond) 1980 Jun;84(3):411–414. doi: 10.1017/s0022172400026930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradbury W. C., Pearson A. D., Marko M. A., Congi R. V., Penner J. L. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol. 1984 Mar;19(3):342–346. doi: 10.1128/jcm.19.3.342-346.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eckhardt T. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978 Sep;1(4):584–588. doi: 10.1016/0147-619x(78)90016-1. [DOI] [PubMed] [Google Scholar]
  6. FREDERICQ P., BETZ-BAREAU M., NICOLLE P. Typage des souches d'Escherichia coli de gastro-entérite infantile par recherche de leurs propiétés colicinogénes. C R Seances Soc Biol Fil. 1956;150(11):2039–2042. [PubMed] [Google Scholar]
  7. Giannella R. A. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. doi: 10.1128/iai.14.1.95-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldschmidt M. C., DuPont H. L. Enteropathogenic Escherichia coli: lack of correlation of serotype with pathogenicity. J Infect Dis. 1976 Feb;133(2):153–156. doi: 10.1093/infdis/133.2.153. [DOI] [PubMed] [Google Scholar]
  9. Kakoyiannis C. K., Winter P. J., Marshall R. B. Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis. Appl Environ Microbiol. 1984 Sep;48(3):545–549. doi: 10.1128/aem.48.3.545-549.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaper J. B., Bradford H. B., Roberts N. C., Falkow S. Molecular epidemiology of Vibrio cholerae in the U.S. Gulf Coast. J Clin Microbiol. 1982 Jul;16(1):129–134. doi: 10.1128/jcm.16.1.129-134.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelly T. J., Jr, Smith H. O. A restriction enzyme from Hemophilus influenzae. II. J Mol Biol. 1970 Jul 28;51(2):393–409. doi: 10.1016/0022-2836(70)90150-6. [DOI] [PubMed] [Google Scholar]
  12. Le Pecq J. B., Paoletti C. A new fluorometric method for RNA and DNA determination. Anal Biochem. 1966 Oct;17(1):100–107. doi: 10.1016/0003-2697(66)90012-1. [DOI] [PubMed] [Google Scholar]
  13. Marshall R. B., Wilton B. E., Robinson A. J. Identification of Leptospira serovars by restriction-endonuclease analysis. J Med Microbiol. 1981 Feb;14(1):163–166. doi: 10.1099/00222615-14-1-163. [DOI] [PubMed] [Google Scholar]
  14. Merson M. H., Rowe B., Black R. E., Huq I., Gross R. J., Eusof A. Use of antisera for identification of enterotoxigenic Escherichia coli. Lancet. 1980 Aug 2;2(8188):222–224. doi: 10.1016/s0140-6736(80)90118-x. [DOI] [PubMed] [Google Scholar]
  15. Nathans D., Smith H. O. Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975;44:273–293. doi: 10.1146/annurev.bi.44.070175.001421. [DOI] [PubMed] [Google Scholar]
  16. Robinson A. J., Ramadass P., Lee A., Marshall R. B. Differentiation of subtypes within Leptospira interrogans serovars Hardjo, Balcanica and Tarassovi, by bacterial restriction-endonuclease DNA analysis (BRENDA). J Med Microbiol. 1982 Aug;15(3):331–338. doi: 10.1099/00222615-15-3-331. [DOI] [PubMed] [Google Scholar]
  17. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sadowski P. L., Peterson B. C., Gerding D. N., Cleary P. P. Physical characterization of ten R plasmids obtained from an outbreak of nosocomial Klebsiella pneumoniae infections. Antimicrob Agents Chemother. 1979 Apr;15(4):616–624. doi: 10.1128/aac.15.4.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Scotland S. M., Day N. P., Cravioto A., Thomas L. V., Rowe B. Production of heat-labile or heat-stable enterotoxins by strains of Escherichia coli belonging to serogroups O44, O114, and O128. Infect Immun. 1981 Jan;31(1):500–503. doi: 10.1128/iai.31.1.500-503.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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