Abstract
Transposon Tn917, carried on plasmid pTV1, was introduced into Bacillus megaterium and transposed efficiently and apparently randomly. Insertional mutations included at least eight different auxotrophic loci, two carbon source loci, and sporulation loci. One trp::Tn917 mutation was further verified as an insertion by both reversion and transduction.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Callahan J. P., Crawford I. P., Hess G. F., Vary P. S. Cotransductional mapping of the trp-his region of Bacillus megaterium. J Bacteriol. 1983 Jun;154(3):1455–1458. doi: 10.1128/jb.154.3.1455-1458.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- English J. D., Vary P. S. Isolation of recombination-defective and UV-sensitive mutants of Bacillus megaterium. J Bacteriol. 1986 Jan;165(1):155–160. doi: 10.1128/jb.165.1.155-160.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fleischer E. R., Vary P. S. Genetic analysis of fusion recombinants and presence of noncomplementing diploids in Bacillus megaterium. J Gen Microbiol. 1985 Apr;131(4):919–926. doi: 10.1099/00221287-131-4-919. [DOI] [PubMed] [Google Scholar]
- Fortnagel P., Freese E. Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle. J Bacteriol. 1968 Apr;95(4):1431–1438. doi: 10.1128/jb.95.4.1431-1438.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garbe J. C., Hess G. F., Franzen M. A., Vary P. S. Genetics of leucine biosynthesis in Bacillus megaterium QM B1551. J Bacteriol. 1984 Feb;157(2):454–459. doi: 10.1128/jb.157.2.454-459.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kleckner N., Chan R. K., Tye B. K., Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. doi: 10.1016/s0022-2836(75)80059-3. [DOI] [PubMed] [Google Scholar]
- LEVINE M. Mutations in the temperate phage P22 and lysogeny in Salmonella. Virology. 1957 Feb;3(1):22–41. doi: 10.1016/0042-6822(57)90021-1. [DOI] [PubMed] [Google Scholar]
- Love P. E., Lyle M. J., Yasbin R. E. DNA-damage-inducible (din) loci are transcriptionally activated in competent Bacillus subtilis. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6201–6205. doi: 10.1073/pnas.82.18.6201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perkins J. B., Youngman P. J. A physical and functional analysis of Tn917, a Streptococcus transposon in the Tn3 family that functions in Bacillus. Plasmid. 1984 Sep;12(2):119–138. doi: 10.1016/0147-619x(84)90058-1. [DOI] [PubMed] [Google Scholar]
- Tomich P. K., An F. Y., Clewell D. B. Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis. J Bacteriol. 1980 Mar;141(3):1366–1374. doi: 10.1128/jb.141.3.1366-1374.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vary P. S. Transduction in Bacillus megaterium. Biochem Biophys Res Commun. 1979 Jun 13;88(3):1119–1124. doi: 10.1016/0006-291x(79)91524-9. [DOI] [PubMed] [Google Scholar]
- Youngman P. J., Perkins J. B., Losick R. Genetic transposition and insertional mutagenesis in Bacillus subtilis with Streptococcus faecalis transposon Tn917. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2305–2309. doi: 10.1073/pnas.80.8.2305. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Youngman P., Perkins J. B., Losick R. A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions. Mol Gen Genet. 1984;195(3):424–433. doi: 10.1007/BF00341443. [DOI] [PubMed] [Google Scholar]
- Youngman P., Perkins J. B., Losick R. Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene. Plasmid. 1984 Jul;12(1):1–9. doi: 10.1016/0147-619x(84)90061-1. [DOI] [PubMed] [Google Scholar]
- Youngman P., Zuber P., Perkins J. B., Sandman K., Igo M., Losick R. New ways to study developmental genes in spore-forming bacteria. Science. 1985 Apr 19;228(4697):285–291. doi: 10.1126/science.228.4697.285. [DOI] [PubMed] [Google Scholar]